ArticleMolecular genetics and genomics : MGG2008
Replacement of a Drosophila Polycomb response element core, and in situ analysis of its DNA motifs.
Article in Molecular genetics and genomics : MGG, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila.Genetics · 2023Article
- Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription.Genes · 2019Review
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Associating cellular epigenetic models with human phenotypes.Nature reviews. Genetics · 2017Review
- Formation of a Polycomb-Domain in the Absence of Strong Polycomb Response Elements.PLoS genetics · 2016Article
- Review
- Combgap contributes to recruitment of Polycomb group proteins in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- What are memories made of? How Polycomb and Trithorax proteins mediate epigenetic memory.Nature reviews. Molecular cell biology · 2014Review
- High-resolution mapping defines the cooperative architecture of Polycomb response elements.Genome research · 2014Article
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- Structural basis for targeting the chromatin repressor Sfmbt to Polycomb response elements.Genes & development · 2013Article
- Polycomb group response elements in Drosophila and vertebrates.Advances in genetics · 2013Review
- Genome-wide polycomb target gene prediction in Drosophila melanogaster.Nucleic acids research · 2012Article
- Quantitative analysis of polycomb response elements (PREs) at identical genomic locations distinguishes contributions of PRE sequence and genomic environment.Epigenetics & chromatin · 2011Article
- Characterization of the polycomb group response elements of the Drosophila melanogaster invected Locus.Molecular and cellular biology · 2010Article
- Functional evolution of cis-regulatory modules at a homeotic gene in Drosophila.PLoS genetics · 2009Article
- Mechanisms of polycomb gene silencing: knowns and unknowns.Nature reviews. Molecular cell biology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-term repression of homeotic genes in the fruit fly is accomplished by proteins of the Polycomb Group, acting at Polycomb response elements (PREs). Here we use gene conversion to mutate specific DNA motifs within a PRE to test their relevance, and we exchange PREs to test their specificity. Previously we showed that removal of a 185 bp core sequence from the bithoraxoid PRE of the bithorax complex results in posteriorly directed segmental transformations. Mutating multiple binding sites for either the PHO or the GAF proteins separately in the core bithoraxoid PRE resulted in only rare and subtle transformations in adult flies. However, when both sets of sites were mutated, the transformations were similar in strength and penetrance to those caused by the deletion of the 185 bp core region. In contrast, mutating the singly occurring binding site of another DNA-binding protein, DSP1 (reportedly essential for PRE-activity), had no similar effect in combination with mutated PHO or GAF sites. Two minimal PREs from other segment-specific regulatory domains of the bithorax complex could substitute for the bithoraxoid PRE core. Our in situ analysis suggests that core PREs are interchangeable, and the cooperation between PHO and GAF binding sites is indispensable for silencing.
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