ArticlePloS one2008
A mouse model of pulmonary metastasis from spontaneous osteosarcoma monitored in vivo by Luciferase imaging.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
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- An improved experimental model of osteosarcoma lung metastases to investigate innovative therapeutic interventions and sex as a biological variable.International journal of pharmaceutics · 2025Article
- The generation and use of animal models of osteosarcoma in cancer research.Genes & diseases · 2024Review
- The p53 tumor suppressor regulates AKR1B1 expression, a metastasis-promoting gene in breast cancer.Frontiers in molecular biosciences · 2023Article
- A Novel Orthotopic Implantation Technique for Osteosarcoma Produces Spontaneous Metastases and Illustrates Dose-Dependent Efficacy of B7-H3-CAR T Cells.Frontiers in immunology · 2021Article
- Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus.Scientific reports · 2019Article
- Positron Emission Tomography DetectsCancer research · 2019Article
- Use of Antimetastatic SOD3-Mimetic Albumin as a Primer in Triple Negative Breast Cancer.Journal of oncology · 2019Article
- Establishment and characterization of in vivo orthotopic bioluminescent xenograft models from human osteosarcoma cell lines in Swiss nude and NSG mice.Cancer medicine · 2018Article
- Highly metastatic K7M2 cell line: A novel murine model capable of in vivo imaging via luciferase vector transfection.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- CORR Insights(®): Do Mesenchymal Stromal Cells Influence Microscopic Residual or Metastatic Osteosarcoma in a Murine Model?Clinical orthopaedics and related research · 2016Article
- Do Mesenchymal Stromal Cells Influence Microscopic Residual or Metastatic Osteosarcoma in a Murine Model?Clinical orthopaedics and related research · 2016Article
- Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment.PloS one · 2016Article
- Modulating mammary tumor growth, metastasis and immunosuppression by siRNA-induced MIF reduction in tumor microenvironment.Cancer gene therapy · 2015Article
- NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma.Oncogene · 2015Article
- Transient SNAIL1 expression is necessary for metastatic competence in breast cancer.Cancer research · 2014Article
- microRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R.International journal of oncology · 2014Article
- Capture and printing of fixed stromal cell membranes for bioactive display on PDMS surfaces.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Biological characterization of preclinical Bioluminescent Osteosarcoma Orthotopic Mouse (BOOM) model: A multi-modality approach.Journal of bone oncology · 2013Article
- MicroRNA-34a inhibits the proliferation and metastasis of osteosarcoma cells both in vitro and in vivo.PloS one · 2012Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteosarcoma (OSA) is lethal when metastatic after chemotherapy and/or surgical treatment. Thus animal models are necessary to study the OSA metastatic spread and to validate novel therapies able to control the systemic disease. We report the development of a syngeneic (Balb/c) murine OSA model, using a cell line derived from a spontaneous murine tumor. METHODOLOGY: The tumorigenic and metastatic ability of OSA cell lines were assayed after orthotopic injection in mice distal femur. Expression profiling was carried out to characterize the parental and metastatic cell lines. Cells from metastases were propagated and engineered to express Luciferase, in order to follow metastases in vivo. PRINCIPAL
findingsLuciferase bioluminescence allowed to monitor the primary tumor growth and revealed the appearance of spontaneous pulmonary metastases. In vivo assays showed that metastasis is a stable property of metastatic OSA cell lines after both propagation in culture and luciferase trasduction. When compared to parental cell line, both unmodified and genetically marked metastatic cells, showed comparable and stable differential expression of the enpp4, pfn2 and prkcd genes, already associated to the metastatic phenotype in human cancer.
conclusionsThis OSA animal model faithfully recapitulates some of the most important features of the human malignancy, such as lung metastatization. Moreover, the non-invasive imaging allows monitoring the tumor progression in living mice. A great asset of this model is the metastatic phenotype, which is a stable property, not modifiable after genetic manipulation.
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