Evidence map›Paper›PMID 18347853›Full record

ArticleCurrent microbiology2008

An easier method for locating replication origins based on real-time polymerase chain reaction.

Y Mei, D Chen, D Sun, Y Yang, Y Huang, P Shen, X Chen

Abstract readComparative StudyEvaluation Study
PubMed Publisher
In one paragraph

Article in Current microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Y MeiState Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
D Chen
D Sun
Y Yang
Y Huang
P Shen
X Chen

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Locating replication origins (ROs) is an old problem to which diverse answers have been previously given. However, a better and more convenient method still remains desirable because previously reported methods are time-consuming. In this study, we report an easier method for locating ROs based on real-time polymerase chain reaction. The feasibility of this method was demonstrated using Escherichia coli K-12 and Halobacterium salinarium NRC-1 as models. Our results show that this method is easy, fast, and accurate in locating ROs.

Indexed as

Replication OriginDNA, ArchaealDNA, BacterialDNA PrimersEscherichia coli K12Halobacterium salinarumPolymerase Chain ReactionSensitivity and SpecificityDNA, ArchaealDNA, BacterialDNA Primers

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.