ArticleArchives of surgery (Chicago, Ill. : 1960)2008
Use of gene expression profiles in cells of peripheral blood to identify new molecular markers of acute pancreatitis.
Article in Archives of surgery (Chicago, Ill. : 1960), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Impact of photobiomodulation therapy on pro-inflammation functionality of human peripheral blood mononuclear cells - a preliminary study.Scientific reports · 2024Article
- Kratom use disorder and unfolded protein response: Evaluating their relationship in a case control study.PloS one · 2023Article
- Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis.International journal of molecular sciences · 2020Article
- Integrating Circulating Immunological and Sputum Biomarkers for the Early Detection of Lung Cancer.Biomarkers in cancer · 2018Article
- A Prediction Model Based on Biomarkers and Clinical Characteristics for Detection of Lung Cancer in Pulmonary Nodules.Translational oncology · 2017Article
- Differential miRNA expressions in peripheral blood mononuclear cells for diagnosis of lung cancer.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Transcriptional profiling of peripheral blood in pancreatic adenocarcinoma patients identifies diagnostic biomarkers.Digestive diseases and sciences · 2014Article
- Functional regulation of phospholipase D expression in cancer and inflammation.The Journal of biological chemistry · 2014Review
- NS5ATP9 mRNA levels in peripheral blood mononuclear cells predict prognosis in patients with gastric cancer.Medical oncology (Northwood, London, England) · 2014Article
- In-depth profiling of the peripheral blood mononuclear cells proteome for clinical blood proteomics.International journal of proteomics · 2014Article
- Phospholipase D1 has a pivotal role in interleukin-1β-driven chronic autoimmune arthritis through regulation of NF-κB, hypoxia-inducible factor 1α, and FoxO3a.Molecular and cellular biology · 2013Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
hypothesisBlood leukocytes play a major role in mediating local and systemic inflammation during acute pancreatitis. We hypothesize that peripheral blood mononuclear cells (PBMCs) in circulation exhibit unique changes in gene expression and could provide a "reporter" function that reflects the inflammatory response in the pancreas with acute pancreatitis.
designTo determine specific changes in blood leukocytes during acute pancreatitis, we studied the gene transcription profile in PBMCs in a rat model of experimental pancreatitis (sodium taurocholate). Normal rats, saline controls, and a model of septic shock were used as a controls. Complementary RNA obtained from PBMCs of each group (n = 3 in each group) were applied to Affymetrix rat genome DNA GeneChip arrays. Main Outcome Measure Changes in gene expression.
resultsFrom the 8799 rat genes analyzed, 140 genes showed unique significant changes in their expression in PBMCs during the acute phase of pancreatitis, but not in sepsis. Among the 140 genes, 57 were up-regulated, while 69 were down-regulated. Platelet-derived growth factor receptor, prostaglandin E(2) receptor, and phospholipase D(1) were among the top up-regulated genes. Others included genes involved in G protein-coupled receptor and transforming growth factor beta-mediated signaling pathways, while genes associated with apoptosis, glucocorticoid receptors, and even the cholecystokinin receptor were down-regulated.
conclusionsMicroarray analysis in transcriptional profiling of PBMCs showed that genes that are uniquely related to molecular and pancreatic function display differential expression in acute pancreatitis. Profiling genes obtained from an easily accessible source during severe pancreatitis may identify surrogate markers for disease severity.
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