ArticleMolecular and cellular biology2008
p110 CUX1 cooperates with E2F transcription factors in the transcriptional activation of cell cycle-regulated genes.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 41 citations in OpenAlex.
- CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex.Cell reports · 2024Article
- Post-GWAS functional analysis identifies CUX1 as a regulator of p16Nature aging · 2022Article
- Identification and characterization of the mediator kinase-dependent myometrial stem cell phosphoproteome.F&S science · 2021Article
- CUT Domain Proteins in DNA Repair and Cancer.Cancers · 2021Review
- CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation.Cancers · 2021Article
- CUX1, A Controversial Player in Tumor Development.Frontiers in oncology · 2020Review
- PIK3CA Is Regulated by CUX1, Promotes Cell Growth and Metastasis in Bladder CancerFrontiers in oncology · 2020Article
- Cux1 regulation of the cyclin kinase inhibitor p27Gene: X · 2019Article
- C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma.Oncotarget · 2017Article
- Article
- KIFC1: a promising chemotherapy target for cancer treatment?Oncotarget · 2016Review
- HSET overexpression fuels tumor progression via centrosome clustering-independent mechanisms in breast cancer patients.Oncotarget · 2015Article
- The transcription factor Cux1 in cerebellar granule cell development and medulloblastoma pathogenesis.Cerebellum (London, England) · 2014Article
- CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers.Nature reviews. Cancer · 2014Review
- Article
- E2F2 and CREB cooperatively regulate transcriptional activity of cell cycle genes.Nucleic acids research · 2013Article
- Genetic basis of MPN: Beyond JAK2-V617F.Current hematologic malignancy reports · 2013Review
- Long-range transcriptional regulation by the p110 CUX1 homeodomain protein on the ENCODE array.BMC genomics · 2013Article
- Transcription factor CUTL1 is a negative regulator of drug resistance in gastric cancer.The Journal of biological chemistry · 2013Article
- Genetic and epigenetic alterations of myeloproliferative disorders.International journal of hematology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor p110 CUX1 was shown to stimulate cell proliferation by accelerating entry into S phase. As p110 CUX1 can function as a transcriptional repressor or activator depending on promoter context, we investigated its mechanism of transcriptional activation using the DNA polymerase alpha gene promoter as a model system. Linker-scanning analysis revealed that a low-affinity E2F binding site is required for transcriptional activation. Moreover, coexpression with a dominant-negative mutant of DP-1 suggested that endogenous E2F factors are indeed needed for p110-mediated activation. Tandem affinity purification, coimmunoprecipitation, chromatin immunoprecipitation, and reporter assays indicated that p110 CUX1 can engage in weak protein-protein interactions with E2F1 and E2F2, stimulate their recruitment to the DNA polymerase alpha gene promoter, and cooperate with these factors in transcriptional activation. On the other hand, in vitro assays suggested that the interaction between CUX1 and E2F1 either is not direct or is regulated by posttranslational modifications. Genome-wide location analysis revealed that targets common to p110 CUX1 and E2F1 included many genes involved in cell cycle, DNA replication, and DNA repair. Comparison of the degree of enrichment for various E2F factors suggested that binding of p110 CUX1 to a promoter will favor the specific recruitment of E2F1, and to a lesser extent E2F2, over E2F3 and E2F4. Reporter assays on a subset of common targets confirmed that p110 CUX1 and E2F1 cooperate in their transcriptional activation. Overall, our results show that p110 CUX1 and E2F1 cooperate in the regulation of many cell cycle genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.