ArticleMolecular and cellular biology2008
The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- Connecting genotype and phenotype in minor spliceosome diseases.RNA (New York, N.Y.) · 2025Review
- Distinct functions for the paralogous RBM41 and U11/U12-65K proteins in the minor spliceosome.Nucleic acids research · 2024Article
- Emerging roles of spliceosome in cancer and immunity.Protein & cell · 2022Review
- Distinct Minor Splicing Patterns across Cancers.Genes · 2022Article
- Field-theoretic density estimation for biological sequence space with applications to 5' splice site diversity and aneuploidy in cancer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Chromosomal instability by mutations in the novel minor spliceosome component CENATAC.The EMBO journal · 2021Article
- Polymerase acidic subunit of H9N2 polymerase complex induces cell apoptosis by binding to PDCD 7 in A549 cells.Virology journal · 2021Article
- The integrity of the U12 snRNA 3' stem-loop is necessary for its overall stability.Nucleic acids research · 2021Article
- At the Intersection of Major and Minor Spliceosomes: Crosstalk Mechanisms and Their Impact on Gene Expression.Frontiers in genetics · 2021Review
- Comprehensive database and evolutionary dynamics of U12-type introns.Nucleic acids research · 2020Article
- New insights into minor splicing-a transcriptomic analysis of cells derived from TALS patients.RNA (New York, N.Y.) · 2019Article
- Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA.RNA (New York, N.Y.) · 2018Article
- The emerging role of minor intron splicing in neurological disorders.Cell stress · 2018Review
- Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.PLoS genetics · 2017Article
- Structural features important for the U12 snRNA binding and minor spliceosome assembly of Arabidopsis U11/U12-small nuclear ribonucleoproteins.RNA biology · 2016Article
- The Arabidopsis homolog of human minor spliceosomal protein U11-48K plays a crucial role in U12 intron splicing and plant development.Journal of experimental botany · 2016Article
- Aberrant splicing of U12-type introns is the hallmark of ZRSR2 mutant myelodysplastic syndrome.Nature communications · 2015Article
- Evolutionarily conserved exon definition interactions with U11 snRNP mediate alternative splicing regulation on U11-48K and U11/U12-65K genes.RNA biology · 2015Article
- Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome.Nucleic acids research · 2014Article
- Minor class splicing shapes the zebrafish transcriptome during development.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is currently known about proteins that make contact with the pre-mRNA in the U12-dependent spliceosome and thereby contribute to intron recognition. Using site-specific cross-linking, we detected an interaction between the U11-48K protein and U12-type 5' splice sites (5'ss). This interaction did not require branch point recognition and was sensitive to 5'ss mutations, suggesting that 48K interacts with the 5'ss during the first steps of prespliceosome assembly in a sequence-dependent manner. RNA interference-induced knockdown of 48K in HeLa cells led to reduced cell growth and the inhibition of U12-type splicing, as well as the activation of cryptic, U2-type splice sites, suggesting that 48K plays a critical role in U12-type intron recognition. 48K knockdown also led to reduced levels of U11/U12 di-snRNP, indicating that 48K contributes to the stability and/or formation of this complex. In addition to making contact with the 5'ss, 48K interacts with the U11-59K protein, a protein at the interface of the U11/U12 di-snRNP. These studies provide important insights into the protein-mediated recognition of the U12-type 5'ss, as well as functionally important interactions within the U11/U12 di-snRNP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.