ArticleInfection and immunity2008
Vibrio parahaemolyticus inhibition of Rho family GTPase activation requires a functional chromosome I type III secretion system.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- The rise of AMPylation: from bacterial beginnings to modern implications in health and disease.Biochemical Society transactions · 2025Review
- Antibiotic Resistance and Characteristics ofMicroorganisms · 2025Article
- Unveiling potential virulence determinants inMicrobiology spectrum · 2024Article
- Genetic Insights into Biofilm Formation by a Pathogenic Strain ofMicroorganisms · 2024Article
- Vibrio variations on a type three theme.Current opinion in microbiology · 2019Review
- The Vibrio alginolyticus T3SS effectors, Val1686 and Val1680, induce cell rounding, apoptosis and lysis of fish epithelial cells.Virulence · 2018Article
- The pathogenesis, detection, and prevention of Vibrio parahaemolyticus.Frontiers in microbiology · 2015Article
- Manipulation of intestinal epithelial cell function by the cell contact-dependent type III secretion systems of Vibrio parahaemolyticus.Frontiers in cellular and infection microbiology · 2014Review
- Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.Frontiers in cellular and infection microbiology · 2014Article
- H-NS is a repressor of major virulence gene loci in Vibrio parahaemolyticus.Frontiers in microbiology · 2014Article
- Vibrio parahaemolyticus ExsE is requisite for initial adhesion and subsequent type III secretion system 1-dependent autophagy in HeLa cells.Microbiology (Reading, England) · 2012Article
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- Transcriptional regulation of opaR, qrr2-4 and aphA by the master quorum-sensing regulator OpaR in Vibrio parahaemolyticus.PloS one · 2012Article
- Bacteria and host interactions in the gut epithelial barrier.Nature chemical biology · 2011Review
- Manipulation of host membranes by bacterial effectors.Nature reviews. Microbiology · 2011Review
- Bacterial protein toxins that modify host regulatory GTPases.Nature reviews. Microbiology · 2011Review
- Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain.The Journal of biological chemistry · 2010Article
- Vibrio VopQ induces PI3-kinase-independent autophagy and antagonizes phagocytosis.Molecular microbiology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio parahaemolyticus is a leading cause of seafood-borne gastroenteritis; however, its virulence mechanisms are not well understood. The identification of type III secreted proteins has provided candidate virulence factors whose functions are still being elucidated. Genotypic strain variability contributes a level of complexity to understanding the role of different virulence factors. The ability of V. parahaemolyticus to inhibit Rho family GTPases and cause cytoskeletal disruption was examined with HeLa cells. After HeLa cells were infected, intracellular Rho activation was inhibited in response to external stimuli. In vitro activation of Rho, Rac, and Cdc42 isolated from infected HeLa cell lysates was also inhibited, indicating that the bacteria were specifically targeting GTPase activation. The inhibition of Rho family GTPase activation was retained for clinical and environmental isolates of V. parahaemolyticus and was dependent on a functional chromosome I type III secretion system (CI-T3SS). GTPase inhibition was independent of hemolytic toxin genotype and the chromasome II (CII)-T3SS. Rho inhibition was accompanied by a shift in the total actin pool to its monomeric form. These phenotypes were abrogated in a mutant strain lacking the CI-T3S effector Vp1686, suggesting that the inhibiting actin polymerization may be a downstream effect of Vp1686-dependent GTPase inhibition. Although Vp1686 has been previously characterized as a potential virulence factor in macrophages, our findings reveal an effect on cultured HeLa cells. The ability to inhibit Rho family GTPases independently of the CII-T3SS and the hemolytic toxins may provide insight into the mechanisms of virulence used by strains lacking these virulence factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.