ArticleNucleic acids research2008
A dual-tag microarray platform for high-performance nucleic acid and protein analyses.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 57 citations in OpenAlex.
- Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A general method for rapid determination of antibiotic susceptibility and species in bacterial infections.Journal of clinical microbiology · 2015Article
- Ratiometric electrochemical proximity assay for sensitive one-step protein detection.Scientific reports · 2014Article
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- Comparison and transfer testing of multiplex ligation detection methods for GM plants.BMC biotechnology · 2012Article
- Profiling cellular protein complexes by proximity ligation with dual tag microarray readout.PloS one · 2012Article
- Selective cell capture and analysis using shallow antibody-coated microchannels.Biomicrofluidics · 2012Article
- Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Multiplex cDNA quantification method that facilitates the standardization of gene expression data.Nucleic acids research · 2011Article
- Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-end.RNA (New York, N.Y.) · 2010Article
- Sensitive plasma protein analysis by microparticle-based proximity ligation assays.Molecular & cellular proteomics : MCP · 2010Article
- Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA.RNA (New York, N.Y.) · 2009Article
- A single molecule array for digital targeted molecular analyses.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA microarrays serve to monitor a wide range of molecular events, but emerging applications like measurements of weakly expressed genes or of proteins and their interaction patterns will require enhanced performance to improve specificity of detection and dynamic range. To further extend the utility of DNA microarray-based approaches we present a high-performance tag microarray procedure that enables probe-based analysis of as little as 100 target cDNA molecules, and with a linear dynamic range close to 10(5). Furthermore, the protocol radically decreases the risk of cross-hybridization on microarrays compared to current approaches, and it also allows for quantification by single-molecule analysis and real-time on-chip monitoring of rolling-circle amplification. We provide proof of concept for microarray-based measurement of both mRNA molecules and of proteins, converted to tag DNA sequences by padlock and proximity probe ligation, respectively.
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