ArticleGene expression patterns : GEP2008
Transcriptional profiling of Wnt4 mutant mouse kidneys identifies genes expressed during nephron formation.
Article in Gene expression patterns : GEP, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Cellular and Molecular Mechanisms of Kidney Development: From the Embryo to the Kidney Organoid.Frontiers in cell and developmental biology · 2020Review
- Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.Development (Cambridge, England) · 2018Article
- A holey pursuit: lumen formation in the developing kidney.Pediatric nephrology (Berlin, Germany) · 2017Review
- Renal dysplasia in the neonate.Current opinion in pediatrics · 2016Review
- Excitatory amino acid transporter 5 is widely expressed in peripheral tissues.European journal of histochemistry : EJH · 2013Article
- A protein kinase A and Wnt-dependent network regulating an intermediate stage in epithelial tubulogenesis during kidney development.Developmental biology · 2012Article
- Article
- Notch pathway activation can replace the requirement for Wnt4 and Wnt9b in mesenchymal-to-epithelial transition of nephron stem cells.Development (Cambridge, England) · 2011Article
- Article
- Characterization of mesonephric development and regeneration using transgenic zebrafish.American journal of physiology. Renal physiology · 2010Article
- Wnt4, the first member of the Wnt family identified in Schistosoma japonicum, regulates worm development by the canonical pathway.Parasitology research · 2010Article
- Molecular anatomy of the kidney: what have we learned from gene expression and functional genomics?Pediatric nephrology (Berlin, Germany) · 2010Review
- Wnt to build a tube: contributions of Wnt signaling to epithelial tubulogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
- An Hh-dependent pathway in lateral plate mesoderm enables the generation of left/right asymmetry.Current biology : CB · 2009Article
- Fras1, a basement membrane-associated protein mutated in Fraser syndrome, mediates both the initiation of the mammalian kidney and the integrity of renal glomeruli.Human molecular genetics · 2008Article
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2 authors at 1 institution in 1 country.
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Abstract
The mature nephron forms from a simple epithelial vesicle into an elaborate structure with distinct regions of specialized physiological function. The molecular components driving the process of nephron development are not well understood. To identify genes that may be informative in this process we conducted a transcriptional profiling screen using Wnt4 mutant kidneys. In Wnt4-/- homozygous mice, condensates and pretubular aggregates are induced, however, epithelial renal vesicles fail to form and subsequent tubulogenesis is blocked. A transcriptional profile comparison between wildtype and Wnt4-/- mutant kidneys at E14.5 was performed using Affymetrix oligonucleotide microarrays to identify nephron-expressed genes. This approach identified 236 genes with expression levels >1.8-fold higher in wildtype versus mutant kidneys, amongst these were a number of known nephron component markers confirming the validity of the screen. These results were further detailed by wholemount in situ hybridization (WISH) of E15.5 urogenital systems (UGS). We annotated the spatial expression pattern of these genes into eight categories of expression. Genes expressed in renal vesicle and their derivatives, structures absent in the mutant, accounted for the largest number of the observed expression patterns. A number of additional genes in areas not directly overlapping the Wnt4 expression domain were also identified including the cap mesenchyme, the collecting duct, and the cortical interstitium. This study provides a useful compendium of molecular markers for the study of nephrogenesis.
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