ArticleNeural development2008
Axonin-1/TAG-1 is required for pathfinding of granule cell axons in the developing cerebellum.
Article in Neural development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Identification of Potential Growth-Related Proteins in Chick Vitreous during Emmetropization Using SWATH-MS and Targeted-Based Proteomics (MRMHR).International journal of molecular sciences · 2024Article
- The Regulatory Roles of Cerebellar Glycosphingolipid Microdomains/Lipid Rafts.International journal of molecular sciences · 2023Review
- Structure and evolution of neuronal wiring receptors and ligands.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Review
- Endoglycan Regulates Purkinje Cell Migration by Balancing Cell-Cell Adhesion.Frontiers in neuroscience · 2022Article
- Live imaging of retinotectal mapping reveals topographic map dynamics and a previously undescribed role for Contactin 2 in map sharpening.Development (Cambridge, England) · 2021Article
- Article
- TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation.Cell reports · 2020Article
- Mitotic granule cell precursors undergo highly dynamic morphological transitions throughout the external germinal layer of the chick cerebellum.Scientific reports · 2019Article
- Contactins in the central nervous system: role in health and disease.Neural regeneration research · 2019Review
- Contactin-2/TAG-1, active on the front line for three decades.Cell adhesion & migration · 2017Article
- Glycosylphosphatidylinositol-Anchored Immunoglobulin Superfamily Cell Adhesion Molecules and Their Role in Neuronal Development and Synapse Regulation.Frontiers in molecular neuroscience · 2017Review
- Manduca Contactin Regulates Amyloid Precursor Protein-Dependent Neuronal Migration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- BACE1 activity regulates cell surface contactin-2 levels.Molecular neurodegeneration · 2014Article
- Axonal patterns and targets of dA1 interneurons in the chick hindbrain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Nuclear factor I and cerebellar granule neuron development: an intrinsic-extrinsic interplay.Cerebellum (London, England) · 2012Review
- Article
- Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.Nucleic acids research · 2011Article
- The effects of Tag-1 on the maturation of mouse cerebellar granule neurons.Cellular and molecular neurobiology · 2011Article
- F3/contactin and TAG1 play antagonistic roles in the regulation of sonic hedgehog-induced cerebellar granule neuron progenitor proliferation.Development (Cambridge, England) · 2011Article
- Neural circuit formation in the cerebellum is controlled by cell adhesion molecules of the Contactin family.Cell adhesion & migrationArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeural development consists of a series of steps, including neurogenesis, patterning, cell migration, axon guidance, and finally, synaptogenesis. Because all these steps proceed in a constantly changing environment, functional gene analyses during development have to take time into account. This is quite challenging, however, as loss-of-function approaches based on classic genetic tools do not allow for the precise temporal control that is required for developmental studies. Gene silencing by RNA interference (RNAi) in combination with the chicken embryo or with cultured embryos opens new possibilities for functional gene analysis in vivo. Axonin-1/TAG-1 is a cell adhesion molecule of the immunoglobulin superfamily with a well defined temporal and spatial expression pattern in the developing vertebrate nervous system. Axonin-1/TAG-1 was shown to promote neurite outgrowth in vitro and to be required for commissural and sensory axon pathfinding in vivo.
resultsTo knock down axonin-1 in a temporally and spatially controlled manner during development of the nervous system, we have combined RNAi with the accessibility of the chicken embryo even at late stages of development. Using ex ovo RNAi, we analyzed the function of axonin-1/TAG-1 in cerebellar development. Axonin-1 is expressed in postmitotic granule cells while they extend their processes, the parallel fibers. In the absence of axonin-1 these processes still extend but no longer in a parallel manner to each other or to the pial surface of the cerebellum.
conclusionAxonin-1/TAG-1 is required for the navigation, but not for the elongation, of granule cell processes in the developing cerebellum in vivo.
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