ArticleAging cell2008
Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity.
Article in Aging cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Werner syndrome exonuclease promotes gut regeneration and causes age-associated gut hyperplasia in Drosophila.PLoS biology · 2025Article
- Dietary restriction fails to extend lifespan of Drosophila model of Werner syndrome.G3 (Bethesda, Md.) · 2024Article
- Characterization of Stress Responses in aBiomolecules · 2021Article
- Gamma irradiation-mediated inactivation of enveloped viruses with conservation of genome integrity: Potential application for SARS-CoV-2 inactivated vaccine development.Open life sciences · 2021Review
- Evidence for premature aging in a Drosophila model of Werner syndrome.Experimental gerontology · 2019Article
- Review
- EXD2 - a new player joins the DSB resection team.Cell cycle (Georgetown, Tex.) · 2016Article
- Lifespan and Stress Resistance in Drosophila with Overexpressed DNA Repair Genes.Scientific reports · 2015Article
- The Drosophila Werner exonuclease participates in an exonuclease-independent response to replication stress.Genetics · 2014Article
- A fluorescence-based exonuclease assay to characterize DmWRNexo, orthologue of human progeroid WRN exonuclease, and its application to other nucleases.Journal of visualized experiments : JoVE · 2013Article
- The Drosophila orthologue of progeroid human WRN exonuclease, DmWRNexo, cleaves replication substrates but is inhibited by uracil or abasic sites : analysis of DmWRNexo activity in vitro.Age (Dordrecht, Netherlands) · 2013Article
- The role of DNA exonucleases in protecting genome stability and their impact on ageing.Age (Dordrecht, Netherlands) · 2012Review
- Drosophila RecQ5 is required for efficient SSA repair and suppression of LOH in vivo.Protein & cell · 2010Article
- Roles of Werner syndrome protein in protection of genome integrity.DNA repair · 2010Review
- dRecQ4 is required for DNA synthesis and essential for cell proliferation in Drosophila.PloS one · 2009Article
- Human RECQL5: guarding the crossroads of DNA replication and transcription and providing backup capability.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The premature human aging Werner syndrome (WS) is caused by mutation of the RecQ-family WRN helicase, which is unique in possessing also 3'-5' exonuclease activity. WS patients show significant genomic instability with elevated cancer incidence. WRN is implicated in restraining illegitimate recombination, especially during DNA replication. Here we identify a Drosophila ortholog of the WRN exonuclease encoded by the CG7670 locus. The predicted DmWRNexo protein shows conservation of structural motifs and key catalytic residues with human WRN exonuclease, but entirely lacks a helicase domain. Insertion of a piggyBac element into the 5' UTR of CG7670 severely reduces gene expression. DmWRNexo mutant flies homozygous for this insertional allele of CG7670 are thus severely hypomorphic; although adults show no gross morphological abnormalities, females are sterile. Like human WS cells, we show that the DmWRNexo mutant flies are hypersensitive to the topoisomerase I inhibitor camptothecin. Furthermore, these mutant flies show highly elevated rates of mitotic DNA recombination resulting from excessive reciprocal exchange. This study identifies a novel WRN ortholog in flies and demonstrates an important role for WRN exonuclease in maintaining genome stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.