ArticleAnalytical chemistry2008
Glucose and lactate biosensors for scanning electrochemical microscopy imaging of single live cells.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 89 citations in OpenAlex.
- Aptamer-Based Scanning Electrochemical Microscopy for Specific and Localized Detection of Analyte Concentration.ACS electrochemistry · 2026Article
- Scanning Electrochemical Impedance Microscopy-Based Assessment of Glucose Biosensors.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Beyond Traditional Synthesis: Electrochemical Approaches to Amine Oxidation for Nitriles and Imines.ACS organic & inorganic Au · 2024Review
- CytochromeProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea.Sensors (Basel, Switzerland) · 2022Review
- A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood.Biosensors · 2021Article
- On-Chip Glucose Detection Based on Glucose Oxidase Immobilized on a Platinum-Modified, Gold Microband Electrode.Biosensors · 2021Article
- Glucose Microsensor with Covalently Immobilized Glucose Oxidase for Probing Bacterial Glucose Uptake by Scanning Electrochemical Microscopy.Analytical chemistry · 2020Article
- Simple Fabrication of Flexible Biosensor Arrays Using Direct Writing for Multianalyte Measurement from Human Astrocytes.SLAS technology · 2020Article
- A multi-sensor system for measuring bovine embryo metabolism.Biosensors & bioelectronics · 2019Article
- First Generation Amperometric Biosensing of Galactose with Xerogel-Carbon Nanotube Layer-By-Layer Assemblies.Nanomaterials (Basel, Switzerland) · 2018Article
- Review
- Biological imaging with scanning electrochemical microscopy.Proceedings. Mathematical, physical, and engineering sciences · 2018Review
- Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems.ACS chemical biology · 2018Review
- A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes.Chemical science · 2018Article
- Electrochemical imaging of cells and tissues.Chemical science · 2018Review
- Droplet Microfluidic Platform for the Determination of Single-Cell Lactate Release.Analytical chemistry · 2016Article
- Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks.Microsystems & nanoengineering · 2016Article
- Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications.Sensors (Basel, Switzerland) · 2015Review
- Multianalyte microphysiometry reveals changes in cellular bioenergetics upon exposure to fluorescent dyes.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have developed glucose and lactate ultramicroelectrode (UME) biosensors based on glucose oxidase and lactate oxidase (with enzymes immobilized onto Pt UMEs by either electropolymerization or casting) for scanning electrochemical microscopy (SECM) and have determined their sensitivity to glucose and lactate, respectively. The results of our evaluations reveal different advantages for sensors constructed by each method: improved sensitivity and shorter manufacturing time for hand-casting, and increased reproducibility for electropolymerization. We have acquired amperometric approach curves (ACs) for each type of manufactured biosensor UME, and these ACs can be used as a means of positioning the UME above a substrate at a known distance. We have used the glucose biosensor UMEs to record profiles of glucose uptake above individual fibroblasts. Likewise, we have employed the lactate biosensor UMEs for recording the lactate production above single cancer cells with the SECM. We also show that oxygen respiration profiles for single cancer cells do not mimic cell topography, but are rather more convoluted, with a higher respiration activity observed at the points where the cell touches the Petri dish. These UME biosensors, along with the application of others already described in the literature, could prove to be powerful tools for mapping metabolic analytes, such as glucose, lactate, and oxygen, in single cancer cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.