ArticleApplied and environmental microbiology2008
Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 77 citations in OpenAlex.
- Recent advances in metabolic engineering for microbial production of L-tyrosine.World journal of microbiology & biotechnology · 2026Review
- Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.Microbial cell factories · 2026Article
- Melanin in fungi: advances in structure, biosynthesis, regulation, and metabolic engineering.Microbial cell factories · 2024Review
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids.Microbial cell factories · 2024Article
- An Efficient Prephenate Dehydrogenase Gene for the Biosynthesis of L-tyrosine: Gene Mining, Sequence Analysis, and Expression Optimization.Foods (Basel, Switzerland) · 2023Article
- Reduction of Lewy Body Pathology by Oral Cinnamon.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2021Article
- GeneReg: a constraint-based approach for design of feasible metabolic engineering strategies at the gene level.Bioinformatics (Oxford, England) · 2021Article
- The entry reaction of the plant shikimate pathway is subjected to highly complex metabolite-mediated regulation.The Plant cell · 2021Article
- BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway.Journal of experimental botany · 2020Article
- Production of Melanins With Recombinant Microorganisms.Frontiers in bioengineering and biotechnology · 2019Review
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Characterization of two key enzymes for aromatic amino acid biosynthesis in symbiotic archaea.Extremophiles : life under extreme conditions · 2016Article
- Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli.Scientific reports · 2015Article
- Production of tyrosine through phenylalanine hydroxylation bypasses the intrinsic feedback inhibition in Escherichia coli.Journal of industrial microbiology & biotechnology · 2015Article
- Production of cinnamic and p-hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli.Microbial cell factories · 2015Article
- Production of Cinnamic and p-Hydroxycinnamic Acids in Engineered Microbes.Frontiers in bioengineering and biotechnology · 2015Review
- Production of salidroside in metabolically engineered Escherichia coli.Scientific reports · 2014Article
- Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.Microbial cell factories · 2014Review
- Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose.Microbial cell factories · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the feedback inhibition-insensitive enzyme cyclohexadienyl dehydrogenase (TyrC) from Zymomonas mobilis and the chorismate mutase domain from native chorismate mutase-prephenate dehydratase (PheA(CM)) from Escherichia coli was compared to the expression of native feedback inhibition-sensitive chorismate mutase-prephenate dehydrogenase (CM-TyrA(p)) with regard to the capacity to produce l-tyrosine in E. coli strains modified to increase the carbon flow to chorismate. Shake flask experiments showed that TyrC increased the yield of l-tyrosine from glucose (Y(l-Tyr/Glc)) by 6.8-fold compared to the yield obtained with CM-TyrA(p). In bioreactor experiments, a strain expressing both TyrC and PheA(CM) produced 3 g/liter of l-tyrosine with a Y(l-Tyr/Glc) of 66 mg/g. These values are 46 and 48% higher than the values for a strain expressing only TyrC. The results show that the feedback inhibition-insensitive enzymes can be employed for strain development as part of a metabolic engineering strategy for l-tyrosine production.
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