ArticleProceedings of the National Academy of Sciences of the United States of America2008
Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 168 papers.
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Who cites it
168 citing papers in PubMed, 359 citations in OpenAlex.
- Enhanced Activity of Glutamine Synthetase by Manipulating a GWAS-Identified BnaA02.GLN1;2 Gene Augments Nitrate Uptake and Yield in Brassica napus.Plant, cell & environment · 2026Article
- HHO5 orchestrates dose-dependent feedback regulation of organic versus inorganic nitrogen signaling in Arabidopsis.The Plant cell · 2026Article
- Article
- Effects of continuous light and skeleton photoperiods on photosynthesis and circadian rhythm in celery.Biotechnology letters · 2026Article
- Time after time: a quarter century of progress in plant circadian biology.Npj biological timing and sleep · 2026Review
- A feedback regulatory loop by MAPK-CCA1 engages auxin signalling to stimulate root foraging for nitrate.Nature plants · 2026Article
- Modelling the short-term response to nitrogen that coordinates events in lateral root initiation.Quantitative plant biology · 2026Article
- Magnesium: An overlooked signalling ion in plant physiology and circadian regulation.Quantitative plant biology · 2026Review
- Model-to-crop conserved NUE Regulons enhance machine learning predictions of nitrogen use efficiency.The Plant cell · 2025Article
- Shining light on Arabidopsis regulatory networks integrating nitrogen use and photosynthesis.The Plant journal : for cell and molecular biology · 2025Article
- StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity.The New phytologist · 2025Article
- The clock-associated LUX ARRHYTHMO regulates high-affinity nitrate transport in Arabidopsis roots.The Plant journal : for cell and molecular biology · 2024Article
- Transcription factor ZmEREB97 regulates nitrate uptake in maize (Zea mays) roots.Plant physiology · 2024Article
- Unlocking Nature's Rhythms: Insights into Secondary Metabolite Modulation by the Circadian Clock.International journal of molecular sciences · 2024Review
- Nitrate Signaling and Its Role in Regulating Flowering Time inInternational journal of molecular sciences · 2024Review
- Integrated Metabolome and Transcriptome Analysis of Gibberellins Mediated the Circadian Rhythm of Leaf Elongation by Regulating Lignin Synthesis in Maize.International journal of molecular sciences · 2024Article
- The Role of Glutamine Synthetase (GS) and Glutamate Synthase (GOGAT) in the Improvement of Nitrogen Use Efficiency in Cereals.Biomolecules · 2023Review
- Improving rice eating and cooking quality by enhancing endogenous expression of a nitrogen-dependent floral regulator.Plant biotechnology journal · 2023Article
- Why did glutamate, GABA, and melatonin become intercellular signalling molecules in plants?eLife · 2023Review
- Merging Signaling with Structure: Functions and Mechanisms of Plant Glutamate Receptor Ion Channels.Annual review of plant biology · 2023Review
108 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Understanding how nutrients affect gene expression will help us to understand the mechanisms controlling plant growth and development as a function of nutrient availability. Nitrate has been shown to serve as a signal for the control of gene expression in Arabidopsis. There is also evidence, on a gene-by-gene basis, that downstream products of nitrogen (N) assimilation such as glutamate (Glu) or glutamine (Gln) might serve as signals of organic N status that in turn regulate gene expression. To identify genome-wide responses to such organic N signals, Arabidopsis seedlings were transiently treated with ammonium nitrate in the presence or absence of MSX, an inhibitor of glutamine synthetase, resulting in a block of Glu/Gln synthesis. Genes that responded to organic N were identified as those whose response to ammonium nitrate treatment was blocked in the presence of MSX. We showed that some genes previously identified to be regulated by nitrate are under the control of an organic N-metabolite. Using an integrated network model of molecular interactions, we uncovered a subnetwork regulated by organic N that included CCA1 and target genes involved in N-assimilation. We validated some of the predicted interactions and showed that regulation of the master clock control gene CCA1 by Glu or a Glu-derived metabolite in turn regulates the expression of key N-assimilatory genes. Phase response curve analysis shows that distinct N-metabolites can advance or delay the CCA1 phase. Regulation of CCA1 by organic N signals may represent a novel input mechanism for N-nutrients to affect plant circadian clock function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.