ArticleClinical nutrition (Edinburgh, Scotland)2008
Amino acids are necessary for the insulin-induced activation of mTOR/S6K1 signaling and protein synthesis in healthy and insulin resistant human skeletal muscle.
Article in Clinical nutrition (Edinburgh, Scotland), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 3 registered trials, which are not on this map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Insulin and Sarcopenia in the Elderly
Nutritional and Contractile Regulation of Muscle Growth (Cycle 2)
ISOLeucine Addition Treatment Effects (ISOLATE) in a Controlled Diet Study
Who cites it
33 citing papers in PubMed, 84 citations in OpenAlex.
- Even effect of milk protein and carbohydrate intake but no further effect of heavy resistance exercise on myofibrillar protein synthesis in older men.European journal of nutrition · 2019Trial
- Soy-Dairy Protein Blend or Whey Protein Isolate Ingestion Induces Similar Postexercise Muscle Mechanistic Target of Rapamycin Complex 1 Signaling and Protein Synthesis Responses in Older Men.The Journal of nutrition · 2016Trial
- Preoperative overnight parenteral nutrition (TPN) improves skeletal muscle protein metabolism indicated by microarray algorithm analyses in a randomized trial.Physiological reports · 2016Trial
- Soy-dairy protein blend and whey protein ingestion after resistance exercise increases amino acid transport and transporter expression in human skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Trial
- Unaffected arm muscle hypercatabolism in dysphagic subacute stroke patients: the effects of essential amino acid supplementation.BioMed research international · 2014Trial
- Protein blend ingestion following resistance exercise promotes human muscle protein synthesis.The Journal of nutrition · 2013Trial
- Novel role of NR1D1 and SLC7A5 in gestational diabetes mellitus: regulation of polyamine metabolism.Journal of diabetes and metabolic disorders · 2025Article
- Polysaccharide Supplements fromBiology · 2025Article
- High-intensity interval training, but not Spirulina supplementation, changes muscle regeneration signaling proteins in aged rats with obesity and diabetes.European journal of translational myology · 2024Article
- Human milk extracellular vesicles enhance muscle growth and physical performance of immature mice associating with Akt/mTOR/p70s6k signaling pathway.Journal of nanobiotechnology · 2023Article
- Role of flavonoids in controlling obesity: molecular targets and mechanisms.Frontiers in nutrition · 2023Review
- The regulatory role of insulin in energy metabolism and leukocyte functions.Journal of leukocyte biology · 2022Review
- A narrative review of skeletal muscle atrophy in critically ill children: pathogenesis and chronic sequelae.Translational pediatrics · 2021Review
- Effect of the lysosomotropic agent chloroquine on mTORC1 activation and protein synthesis in human skeletal muscle.Nutrition & metabolism · 2021Article
- Repurposing Tranexamic Acid as an Anticancer Agent.Frontiers in pharmacology · 2021Article
- End-Stage Renal Disease Patients Lose a Substantial Amount of Amino Acids during Hemodialysis.The Journal of nutrition · 2020Article
- Molecular regulation of human skeletal muscle protein synthesis in response to exercise and nutrients: a compass for overcoming age-related anabolic resistance.American journal of physiology. Cell physiology · 2019Review
- Role of Nutrition and Muscle in Cirrhosis.Current treatment options in gastroenterology · 2016Review
- Maternal low-protein diet affects myostatin signaling and protein synthesis in skeletal muscle of offspring piglets at weaning stage.European journal of nutrition · 2015Article
- A metabolic link to skeletal muscle wasting and regeneration.Frontiers in physiology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAmino acids (AA) activate the mammalian target of rapamycin (mTOR) signaling pathway but overactivation has a negative feedback effect on insulin signaling which may lead to insulin resistance and type 2 diabetes (T2DM). PURPOSE: To determine the effect of reduced AA concentrations on mTOR and insulin signaling during increased nutrient and insulin availability.
methodsSix control and six T2DM subjects were studied at baseline and following a 5h AA lowering high energy and insulin clamp. Stable isotopic techniques in combination with femoral catheterizations were used to measure AA kinetics across the leg while muscle biopsies were used to measure mTOR and insulin signaling proteins using immunoblotting techniques.
resultsAA concentrations decreased by approximately 30-60% in both groups (p<0.05). Phospho-mTOR, S6K1, eEF2, and eIF2alpha were unchanged in both groups following the clamp (p>0.05). In T2DM subjects, IRS-1 serine phosphorylation was unchanged while phospho-AMPKalpha decreased and phospho-Akt, phospho-AS160 and glucose uptake increased following the clamp (p<0.05). In comparison, AA concentrations were maintained in a separate group during an insulin infusion. In this group, phospho-Akt, mTOR and S6K1 (n=4) increased.
conclusionAmino acids are necessary for insulin-induced activation of mTOR signaling and protein synthesis in both healthy and insulin resistant skeletal muscle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.