Evidence map›Paper›PMID 18341300›Full record

ArticleNano letters2008

Single-cell fluorescence imaging using metal plasmon-coupled probe 2: single-molecule counting on lifetime image.

Jian Zhang, Yi Fu, Dong Liang, Kazik Nowaczyk, Richard Y Zhao, Joseph R Lakowicz

Abstract read
In one paragraph

Article in Nano letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Fluorescence Intensity and Lifetime Cell Imaging with Luminescent Gold Nanoclusters.The journal of physical chemistry. C, Nanomaterials and interfaces · 2012
    Article
  5. Article
  6. Fluorescent metal nanoshell and CK19 detection on single cell image.Biochemical and biophysical research communications · 2011
    Article
  7. Review
  8. Article
  9. Fluorescent Metal Nanoshells: Lifetime-Tunable Molecular Probes in Fluorescent Cell Imaging.The journal of physical chemistry. C, Nanomaterials and interfaces · 2011
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Enhanced fluorescence images for labeled cells on silver island films.Langmuir : the ACS journal of surfaces and colloids · 2008
    Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jian ZhangCenter for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.
Yi Fu
Dong Liang
Kazik Nowaczyk
Richard Y Zhao
Joseph R Lakowicz

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Metallic Surfaces and Particles in DNA AnalysisR01HG002655 · NHGRI · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2003 to 2012
$2.8M
SEQUENCE-SPECIFIC CHEMICAL NUCLEASES FOR GENOME ANALYSISR01HG000255 · NHGRI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SIGMAN, DAVID S · 1991 to 1993
–
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NHGRI NIH HHS HG-00255NHGRI NIH HHS R01 HG002655
6 · The paper itself

Abstract

Multiple Alexa Fluor 647-conjugated concanavalin A (conA) molecules were covalently bound to a single 20 nm silver particle to synthesize metal plasmon-coupled probes (PCPs). The fluorescence images were recorded by scanning confocal microscopy in both intensity and lifetime. The brightness of PCPs was 30-fold brighter than those of free conA and the lifetime of PCPs was shortened dramatically. PCPs were used to label T-lymphocytic ( PM1) cells. The emission spots by PCPs bound on the cell surfaces were separated clearly from the cell images by autofluorescence due to the brighter signal and shorter lifetime of PCPs. The emission spots by PCPs were also scanned in three dimensions to count the distribution of bound fluorophores on the cell surfaces. The metal-associated fluorophores thus are suggested using as novel molecular imaging agents to quantify the components and describe their distributions on the cell surfaces.

Indexed as

Molecular ProbesFluorescenceMetalsMicroscopy, ConfocalMetalsMolecular Probes

Identifiers

PMID18341300
PMCPMC2739996

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.