ArticleBiophysical journal2008
Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Cationic Polyelectrolyte Adsorption onto Anionic Nanoparticles Analyzed with Frequency-Domain Scanning Fluorescence Correlation Spectroscopy.Small methods · 2025Article
- Quantification of membrane fluidity in bacteria using TIR-FCS.Biophysical journal · 2024Article
- Quantifying spatial and temporal variations of the cell membrane ultra-structure by bimFCS.Methods (San Diego, Calif.) · 2018Review
- Simultaneous Surface-Near and Solution Fluorescence Correlation Spectroscopy.Journal of fluorescence · 2016Article
- Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy.Biomedical optics express · 2016Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- Article
- Total internal reflection fluorescence microscopy imaging-guided confocal single-molecule fluorescence spectroscopy.The Review of scientific instruments · 2012Article
- Fluorescence techniques to study lipid dynamics.Cold Spring Harbor perspectives in biology · 2011Review
- Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications.Applied spectroscopy · 2011Review
- Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.International journal of molecular sciences · 2010Review
- Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy.Biophysical journal · 2009Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Fluorescence fluctuation spectroscopy: ushering in a new age of enlightenment for cellular dynamics.Biophysical reviews · 2009Article
- Accurate determination of membrane dynamics with line-scan FCS.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence correlation spectroscopy with total internal reflection excitation (TIR-FCS) is a promising method with emerging biological applications for measuring binding dynamics of fluorescent molecules to a planar substrate as well as diffusion coefficients and concentrations at the interface. Models for correlation functions proposed so far are rather approximate for most conditions, since they neglect lateral diffusion of fluorophores. Here we propose accurate extensions of previously published models for axial correlation functions taking into account lateral diffusion through detection profiles realized in typical experiments. In addition, we consider the effects of surface-generated emission in objective-based TIR-FCS. The expressions for correlation functions presented here will facilitate quantitative and accurate measurements with TIR-FCS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.