ArticleBiophysical journal2008
Actin cytoskeleton-dependent dynamics of the human serotonin1A receptor correlates with receptor signaling.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 71 citations in OpenAlex.
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- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.bioRxiv : the preprint server for biology · 2023Article
- Integrity of the Actin Cytoskeleton of Host Macrophages is Necessary for Mycobacterial Entry.The Journal of membrane biology · 2022Review
- Chronic cholesterol depletion increases F-actin levels and induces cytoskeletal reorganization via a dual mechanism.Journal of lipid research · 2022Article
- Role of Actin Cytoskeleton in Dynamics and Function of the SerotoninBiophysical journal · 2020Article
- Lateral diffusion and signaling of receptor for advanced glycation end-products (RAGE): a receptor involved in chronic inflammation.European biophysics journal : EBJ · 2018Article
- Methods used to study the oligomeric structure of G-protein-coupled receptors.Bioscience reports · 2017Review
- Cholesterol-dependent Conformational Plasticity in GPCR Dimers.Scientific reports · 2016Article
- Spatiotemporal Control of Transmembrane Proteins through the Cytoskeleton: An Evolving Story.Biophysical journal · 2016Article
- Dynamics of the actin cytoskeleton mediates receptor cross talk: An emerging concept in tuning receptor signaling.The Journal of cell biology · 2016Review
- Effect of Spatial Inhomogeneities on the Membrane Surface on Receptor Dimerization and Signal Initiation.Frontiers in cell and developmental biology · 2016Article
- Article
- Atomistic Monte Carlo simulation of lipid membranes.International journal of molecular sciences · 2014Review
- A toolbox of fluorescence microscopic approaches reveals dynamics and assembly of a membrane-associated protein.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyzing the dynamics of membrane proteins in the context of cellular signaling represents a challenging problem in contemporary cell biology. Lateral diffusion of lipids and proteins in the cell membrane is known to be influenced by the cytoskeleton. In this work, we explored the role of the actin cytoskeleton on the mobility of the serotonin(1A) (5-HT(1A)) receptor, stably expressed in CHO cells, and its implications in signaling. FRAP analysis of 5-HT(1A)R-EYFP shows that destabilization of the actin cytoskeleton induced by either CD or elevation of cAMP levels mediated by forskolin results in an increase in the mobile fraction of the receptor. The increase in the mobile fraction is accompanied by a corresponding increase in the signaling efficiency of the receptor. Interestingly, with increasing concentrations of CD used, the increase in the mobile fraction exhibited a correlation of approximately 0.95 with the efficiency in ligand-mediated signaling of the receptor. Radioligand binding and G-protein coupling of the receptor were found to be unaffected upon treatment with CD. Our results suggest that signaling by the serotonin(1A) receptor is correlated with receptor mobility, implying thereby that the actin cytoskeleton could play a regulatory role in receptor signaling. These results may have potential significance in the context of signaling by GPCRs in general and in the understanding of GPCR-cytoskeleton interactions with respect to receptor signaling in particular.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.