ArticleBiophysical journal2008
Nonequilibrium single molecule protein folding in a coaxial mixer.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Sample-minimizing co-flow cell for time-resolved pump-probe X-ray solution scattering.Journal of synchrotron radiation · 2023Article
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- AnThe Journal of biological chemistry · 2017Article
- Shedding light on protein folding landscapes by single-molecule fluorescence.Chemical Society reviews · 2014Review
- A simple three-dimensional-focusing, continuous-flow mixer for the study of fast protein dynamics.Lab on a chip · 2013Article
- Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes.Nature protocols · 2013Article
- Single-molecule FRET of protein structure and dynamics - a primer.Journal of nanobiotechnology · 2013Article
- Nanochannel-based single molecule recycling.Nano letters · 2012Article
- How, when and why proteins collapse: the relation to folding.Current opinion in structural biology · 2012Review
- Protein folding at single-molecule resolution.Biochimica et biophysica acta · 2011Review
- Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing.Nature methods · 2011Article
- High-throughput single-molecule optofluidic analysis.Nature methods · 2011Article
- Quantifying heterogeneity and conformational dynamics from single molecule FRET of diffusing molecules: recurrence analysis of single particles (RASP).Physical chemistry chemical physics : PCCP · 2011Article
- Multicolor single-molecule FRET to explore protein folding and binding.Molecular bioSystems · 2010Review
- Microfluidic device for single-molecule experiments with enhanced photostability.Journal of the American Chemical Society · 2009Article
- Collapse transition in proteins.Physical chemistry chemical physics : PCCP · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a continuous-flow mixing device suitable for monitoring bioconformational reactions at the single-molecule level with a response time of approximately 10 ms under single-molecule flow conditions. Its coaxial geometry allows three-dimensional hydrodynamic focusing of sample fluids to diffraction-limited dimensions where diffusional mixing is rapid and efficient. The capillary-based design enables rapid in-lab construction of mixers without the need for expensive lithography-based microfabrication facilities. In-line filtering of sample fluids using granulated silica particles virtually eliminates clogging and extends the lifetime of each device to many months. In this article, to determine both the distance-to-time transfer function and the instrument response function of the device we characterize its fluid flow and mixing properties using both fluorescence cross-correlation spectroscopy velocimetry and finite element fluid dynamics simulations. We then apply the mixer to single molecule FRET protein folding studies of Chymotrypsin Inhibitor protein 2. By transiently populating the unfolded state of Chymotrypsin Inhibitor Protein 2 (CI2) under nonequilibrium in vitro refolding conditions, we spatially and temporally resolve the denaturant-dependent nonspecific collapse of the unfolded state from the barrier-limited folding transition of CI2. Our results are consistent with previous CI2 mixing results that found evidence for a heterogeneous unfolded state consisting of cis- and trans-proline conformers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.