ArticleBiophysical journal2008
Direct visualization of spruce budworm antifreeze protein interacting with ice crystals: basal plane affinity confers hyperactivity.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 116 citations in OpenAlex.
- Application of Antifreeze Substances in Food Cryopreservation.Foods (Basel, Switzerland) · 2025Review
- Engineered ice-binding protein (FfIBP) shows increased stability and resistance to thermal and chemical denaturation compared to the wildtype.Scientific reports · 2024Article
- The Spruce Budworm Genome: Reconstructing the Evolutionary History of Antifreeze Proteins.Genome biology and evolution · 2022Article
- High Water Density at Non-Ice-Binding Surfaces Contributes to the Hyperactivity of Antifreeze Proteins.The journal of physical chemistry letters · 2021Article
- A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering.3 Biotech · 2019Review
- Protein/Ice Interaction: High-Resolution Synchrotron X-ray Diffraction Differentiates Pharmaceutical Proteins from Lysozyme.The journal of physical chemistry. B · 2019Article
- Laboratory-Scale Isolation of Insect Antifreeze Protein for Cryobiology.Biomolecules · 2019Article
- Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structures, dynamics, and hydrogen-bond interactions of antifreeze proteins in TIP4P/Ice water and their dependence on force fields.PloS one · 2018Article
- Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.Marine drugs · 2017Review
- Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana.Plant biotechnology journal · 2017Article
- Article
- Microfluidic Cold-Finger Device for the Investigation of Ice-Binding Proteins.Biophysical journal · 2016Article
- Putting life on ice: bacteria that bind to frozen water.Journal of the Royal Society, Interface · 2016Article
- Flies expand the repertoire of protein structures that bind ice.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Ice-binding proteins that accumulate on different ice crystal planes produce distinct thermal hysteresis dynamics.Journal of the Royal Society, Interface · 2014Article
- Recombinant Dendroides canadensis antifreeze proteins as potential ingredients in cryopreservation solutions.Cryobiology · 2014Article
- Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity.Journal of visualized experiments : JoVE · 2014Article
- Inhibition of ice growth and recrystallization by zirconium acetate and zirconium acetate hydroxide.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antifreeze proteins (AFPs) protect certain organisms from freezing by adhering to ice crystals, thereby preventing their growth. All AFPs depress the nonequilibrium freezing temperature below the melting point; however AFPs from overwintering insects, such as the spruce budworm (sbw) are 10-100 times more effective than most fish AFPs. It has been proposed that the exceptional activity of these AFPs depends on their ability to prevent ice growth at the basal plane. To test the hypothesis that the hyperactivity of sbwAFP results from direct affinity to the basal plane, we fluorescently tagged sbwAFP and visualized it on the surface of ice crystals using fluorescence microscopy. SbwAFP accumulated at the six prism plane corners and the two basal planes of hexagonal ice crystals. In contrast, fluorescently tagged fish type III AFP did not adhere to the basal planes of a single-crystal ice hemisphere. When ice crystals were grown in the presence of a mixture of type III AFP and sbwAFP, a hybrid crystal shape was produced with sbwAFP bound to the basal planes of truncated bipyramidal crystals. These observations are consistent with the blockage of c-axial growth of ice as a result of direct interaction of sbwAFP with the basal planes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.