ArticleBiophysical journal2008
Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 119 citations in OpenAlex.
- Cholesterol, PtdIns(4,5)PbioRxiv : the preprint server for biology · 2026Article
- Single-molecule imaging quantifies oncogenic KRAS dynamics for enhanced accuracy of therapeutic efficacy assessment.iScience · 2025Article
- Cancer cells suppress NK cell activity by actin-driven polarization of inhibitory ligands to the immunological synapse.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Recent Advancements in Imaging Techniques for Individual Extracellular Vesicles.Molecules (Basel, Switzerland) · 2024Review
- A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane.Scientific reports · 2024Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging.Glycoconjugate journal · 2023Review
- The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane.Membranes · 2023Review
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion Data.F1000Research · 2021Article
- DARC, Glycophorin A, Band 3, and GLUT1 Diffusion in Erythrocytes: Insights into Membrane Complexes.Biophysical journal · 2020Article
- AMPA receptors in the synapse turnover by monomer diffusion.Nature communications · 2019Article
- Mapping the stochastic sequence of individual ligand-receptor binding events to cellular activation: T cells act on the rare events.Science signaling · 2019Article
- Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane.iScience · 2018Article
- Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells.Journal of experimental botany · 2018Article
- A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking.Biophysical journal · 2017Article
- Dynamic Meso-Scale Anchorage of GPI-Anchored Receptors in the Plasma Membrane: Prion Protein vs. Thy1.Cell biochemistry and biophysics · 2017Article
- GPI-anchored proteins are confined in subdiffraction clusters at the apical surface of polarized epithelial cells.The Biochemical journal · 2017Article
- Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state.Nature communications · 2017Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, investigations using single-fluorescent-molecule tracking at frame rates of up to 65 Hz, showed that the transmembrane MHC class II protein and its GPI-anchored modified form expressed in CHO cells undergo simple Brownian diffusion, without any influence of actin depolymerization with cytochalasin D. These results are at apparent variance with the view that GPI-anchored proteins stay with cholesterol-enriched raft domains, as well as with the observation that both lipids and transmembrane proteins undergo short-term confined diffusion within a compartment and long-term hop diffusion between compartments. Here, this apparent discrepancy has been resolved by reexamining the same paradigm, by using both high-speed single-particle tracking (50 kHz) and single fluorescent-molecule tracking (30 Hz). Both molecules exhibited rapid hop diffusion between 40-nm compartments, with an average dwell time of 1-3 ms in each compartment. Cytochalasin D hardly affected the hop diffusion, consistent with previous observations, whereas latrunculin A increased the compartment sizes with concomitant decreases of the hop rates, which led to an approximately 50% increase in the median macroscopic diffusion coefficient. These results indicate that the actin-based membrane skeleton influences the diffusion of both transmembrane and GPI-anchored proteins.
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