ArticleThe Journal of biological chemistry2008
The Med1 subunit of transcriptional mediator plays a central role in regulating CCAAT/enhancer-binding protein-beta-driven transcription in response to interferon-gamma.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 38 citations in OpenAlex.
- CpG content contributes to tissue-resident macrophage enhancer selection.Nucleic acids research · 2026Article
- Characterization ofToxins · 2023Article
- Critical roles of transcriptional coactivator MED1 in the formation and function of mouse adipose tissues.Genes & development · 2021Article
- Evidence for additive and synergistic action of mammalian enhancers during cell fate determination.eLife · 2021Article
- MED1 Deficiency in Macrophages Accelerates Intimal Hyperplasia via ROS Generation and Inflammation.Oxidative medicine and cellular longevity · 2021Article
- CDK8-Novel Therapeutic Opportunities.Pharmaceuticals (Basel, Switzerland) · 2019Review
- Essential role of MED1 in the transcriptional regulation of ER-dependent oncogenic miRNAs in breast cancer.Scientific reports · 2018Article
- Mediator 1 Is Atherosclerosis Protective by Regulating Macrophage Polarization.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Cyclin C regulates adipogenesis by stimulating transcriptional activity of CCAAT/enhancer-binding protein α.The Journal of biological chemistry · 2017Article
- Role of lncRNAs in Cellular Aging.Frontiers in endocrinology · 2016Review
- PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional program.Genes & development · 2015Article
- A functional N-terminal domain in C/EBPβ-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.Journal of cellular physiology · 2014Article
- Med1 subunit of the mediator complex in nuclear receptor-regulated energy metabolism, liver regeneration, and hepatocarcinogenesis.Gene expression · 2014Review
- CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.Genes to cells : devoted to molecular & cellular mechanisms · 2014Article
- Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes.The Journal of biological chemistry · 2013Article
- Mediator subunits MED1 and MED24 cooperatively contribute to pubertal mammary gland development and growth of breast carcinoma cells.Molecular and cellular biology · 2012Article
- Chromatin immunoprecipitation assay as a tool for analyzing transcription factor activity.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Arc of a vicious circle: pathways activated by Mycobacterium tuberculosis that target the HIV-1 long terminal repeat.American journal of respiratory cell and molecular biology · 2011Review
- Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein β.Journal of neuroinflammation · 2011Article
- C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells.Journal of cellular physiology · 2011Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Transcription factor CCAAT/enhancer-binding protein (C/EBP)-beta is crucial for regulating transcription of genes involved in a number of diverse cellular processes, including those involved in some cytokine-induced responses. However, the mechanisms that contribute to its diverse transcriptional activity are not yet fully understood. To gain an understanding into its mechanisms of action, we took a proteomic approach and identified cellular proteins that associate with C/EBP-beta in an interferon (IFN)-gamma-dependent manner. Transcriptional mediator (Mediator) is a multisubunit protein complex that regulates signal-induced cellular gene transcription from enhancer-bound transcription factor(s). Here, we report that the Med1 subunit of the Mediator as a C/EBP-beta-interacting protein. Using gene knock-out cells and mutational and RNA interference approaches, we show that Med1 is critical for IFN-induced expression of certain genes. Med1 associates with C/EBP-beta through a domain located between amino acids 125 and 155 of its N terminus. We also show that the MAPK, ERK1/2, and an ERK phosphorylation site within regulatory domain 2, more specifically the Thr(189) residue, of C/EBP-beta are essential for it to bind to Med1. Last, an ERK-regulated site in Med1 protein is also essential for up-regulating IFN-induced transcription although not critical for binding to C/EBP-beta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.