ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2008
Nanobiomechanics of proteins and biomembrane.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 20 citations in OpenAlex.
- Nanomechanics of HaloTag tethers.Journal of the American Chemical Society · 2013Article
- Compliance profile of the human cornea as measured by atomic force microscopy.Micron (Oxford, England : 1993) · 2012Article
- Elastic modulus determination of normal and glaucomatous human trabecular meshwork.Investigative ophthalmology & visual science · 2011Article
- The applications of atomic force microscopy to vision science.Investigative ophthalmology & visual science · 2010Review
- Introduction. Japan: its tradition and hot topics in biological sciences.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Article
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Authors and funding
1 author at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A review of the work done in the Laboratory of Biodynamics of Tokyo Institute of Technology in the last decade has been summarized in this article in relation to the results reported from other laboratories. The emphasis here is the application of nanomechanics based on the force mode of atomic force microscopy (AFM) to proteins and protein-based biological structures. Globular proteins were stretched in various ways to detect the localized rigidity inside of the molecule. When studied by this method, bovine carbonic anhydrase II (BCA II), calmodulin and OspA protein all showed the presence of localized rigid structures inside the molecules. Protein compression experiments were done on BCA II to obtain an estimate of the Young modulus and its change in the process of denaturation. Then, the AFM probe method was turned on to cell membranes and cytoplasmic components. Force curves accompanying the extraction process of membrane proteins from intact cells were analysed in relation to their interaction with the cytoskeletal components. By pushing the AFM probe further into the cytoplasm, mRNAs were recovered from a live cell with minimal damage, and multiplied using PCR technology for their identification. Altogether, the work introduced here forms the basis of nanomechanics of protein and protein-based biostructures and application of the nanomechanical technology to cell biology.
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