ArticleMolecular biology of the cell2008
Lectin-deficient calreticulin retains full functionality as a chaperone for class I histocompatibility molecules.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction.Developmental biology · 2017Article
- High-level secretion of native recombinant human calreticulin in yeast.Microbial cell factories · 2015Article
- Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Calreticulin in the immune system: ins and outs.Trends in immunology · 2013Review
- RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.BMC genomics · 2012Article
- Structural and functional relationships between the lectin and arm domains of calreticulin.The Journal of biological chemistry · 2011Article
- The Ca2+ pumps of the endoplasmic reticulum and Golgi apparatus.Cold Spring Harbor perspectives in biology · 2011Review
- Essential glycan-dependent interactions optimize MHC class I peptide loading.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- X-ray structure of the human calreticulin globular domain reveals a peptide-binding area and suggests a multi-molecular mechanism.PloS one · 2011Article
- Calsperin is a testis-specific chaperone required for sperm fertility.The Journal of biological chemistry · 2011Article
- Distinct functions for the glycans of tapasin and heavy chains in the assembly of MHC class I molecules.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- The polypeptide binding conformation of calreticulin facilitates its cell-surface expression under conditions of endoplasmic reticulum stress.The Journal of biological chemistry · 2011Article
- ER Stress and Iron Homeostasis: A New Frontier for the UPR.Biochemistry research international · 2011Article
- Calreticulin controls the rate of assembly of CD1d molecules in the endoplasmic reticulum.The Journal of biological chemistry · 2010Article
- Functional relationship between protein disulfide isomerase family members during the oxidative folding of human secretory proteins.Molecular biology of the cell · 2010Article
- Glycoproteomic analysis of embryonic stem cells: identification of potential glycobiomarkers using lectin affinity chromatography of glycopeptides.Journal of proteome research · 2010Article
- Modes of calreticulin recruitment to the major histocompatibility complex class I assembly pathway.The Journal of biological chemistry · 2010Article
- Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules.The EMBO journal · 2009Article
- Intracellular assembly and trafficking of MHC class I molecules.Traffic (Copenhagen, Denmark) · 2009Review
- ERp57 does not require interactions with calnexin and calreticulin to promote assembly of class I histocompatibility molecules, and it enhances peptide loading independently of its redox activity.The Journal of biological chemistry · 2009Article
Corrections and comments
- Retraction · 2016-08-01Contamination of Cell Lines/Tissues · Results Not Reproducible ·
- Retracted
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Calreticulin is a molecular chaperone of the endoplasmic reticulum that uses both a lectin site specific for Glc(1)Man(5-9)GlcNAc(2) oligosaccharides and a polypeptide binding site to interact with nascent glycoproteins. The latter mode of substrate recognition is controversial. To examine the relevance of polypeptide binding to protein folding in living cells, we prepared lectin-deficient mutants of calreticulin and examined their abilities to support the assembly and quality control of mouse class I histocompatibility molecules. In cells lacking calreticulin, class I molecules exhibit inefficient loading of peptide ligands, reduced cell surface expression and aberrantly rapid export from the endoplasmic reticulum. Remarkably, expression of calreticulin mutants that are completely devoid of lectin function fully complemented all of the class I biosynthetic defects. We conclude that calreticulin can use nonlectin-based modes of substrate interaction to effect its chaperone and quality control functions on class I molecules in living cells. Furthermore, pulse-chase coimmunoisolation experiments revealed that lectin-deficient calreticulin bound to a similar spectrum of client proteins as wild-type calreticulin and dissociated with similar kinetics, suggesting that lectin-independent interactions are commonplace in cells and that they seem to be regulated during client protein maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.