ArticleJournal of clinical microbiology2008
Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: clinical application for diagnosis of tuberculous meningitis.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis.Systematic reviews · 2017Pooled it
- Development and application of a rapid Mycobacterium tuberculosis detection technique using polymerase spiral reaction.Scientific reports · 2018Article
- Optic disc swelling in a patient with tuberculous meningitis: a diagnostic challenge.BMJ case reports · 2017Article
- Comparison of DNA Extraction Protocols and Molecular Targets to Diagnose Tuberculous Meningitis.Tuberculosis research and treatment · 2017Article
- Real time PCR in childhood tuberculosis: a valuable diagnostic tool.Indian journal of pediatrics · 2015Article
- A possible complementary tool for diagnosing tuberculosis: a feasibility test of immunohistochemical markers.International journal of clinical and experimental pathology · 2015Article
- Microbial DNA testing for inflammatory diseases of the brain of uncertain etiology.Neurology. Clinical practice · 2014Article
- The PCR-Based Diagnosis of Central Nervous System Tuberculosis: Up to Date.Tuberculosis research and treatment · 2012Article
- Cyclic nucleotide phosphodiesterase 7B mRNA: an unfavorable characteristic in chronic lymphocytic leukemia.International journal of cancer · 2011Article
- Loop-mediated isothermal amplification for rapid and reliable diagnosis of tuberculous meningitis.Journal of clinical microbiology · 2011Article
- Approach to the diagnosis and management of tuberculous meningitis.Current infectious disease reports · 2010Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the "gold standard" for diagnosis of tuberculous meningitis (TBM) is bacterial isolation of Mycobacterium tuberculosis, there are still several complex issues. Recently, we developed an internally controlled novel wide-range quantitative nested real-time PCR (WR-QNRT-PCR) assay for M. tuberculosis DNA in order to rapidly diagnose TBM. For use as an internal control calibrator to measure the copy number of M. tuberculosis DNA, an original new-mutation plasmid (NM-plasmid) was developed. Due to the development of the NM-plasmid, the WR-QNRT-PCR assay demonstrated statistically significant accuracy over a wide detection range (1 to 10(5) copies). In clinical applications, the WR-QNRT-PCR assay revealed sufficiently high sensitivity (95.8%) and specificity (100%) for 24 clinically suspected TBM patients. In conditional logistic regression analysis, a copy number of M. tuberculosis DNA (per 1 ml of cerebrospinal fluid) of >8,000 was an independent risk factor for poor prognosis for TBM (i.e., death) (odds ratio, 16.142; 95% confidence interval, 1.191 to 218.79; P value, 0.0365). In addition, the copy numbers demonstrated by analysis of variance statistically significant alterations (P < 0.01) during the clinical treatment course for 10 suspected TBM patients. In simple regression analysis, the significant correlation (R(2) = 0.597; P < 0.0001) was demonstrated between copy number and clinical stage of TBM. We consider the WR-QNRT-PCR assay to be a useful and advanced assay technique for assessing the clinical treatment course of TBM.
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