ArticlePLoS biology2008
Creatine kinase-mediated ATP supply fuels actin-based events in phagocytosis.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 75 citations in OpenAlex.
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- Bioenergetic-active hybrid nanovesicles empower tumor associated macrophages phagocytosis for cancer immunotherapy.Materials today. Bio · 2026Article
- Arginine metabolism in myeloid cells in health and disease.Seminars in immunopathology · 2025Review
- Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype.Theranostics · 2024Article
- Fundamental role for the creatine kinase pathway in protection from murine colitis.Mucosal immunology · 2023Article
- ATP Consumption Is Coupled with Endocytosis in Exudated Neutrophils.International journal of molecular sciences · 2023Article
- Changes in nanomechanical properties of single neuroblastoma cells as a model for oxygen and glucose deprivation (OGD).Scientific reports · 2022Article
- Direct modulation of microglial function by electrical field.Frontiers in cell and developmental biology · 2022Article
- Metabolic Basis of Creatine in Health and Disease: A Bioinformatics-Assisted Review.Nutrients · 2021Review
- An RNAi screen of the kinome in epithelial follicle cells of the Drosophila melanogaster ovary reveals genes required for proper germline death and clearance.G3 (Bethesda, Md.) · 2021Article
- Creatine Transporter, Reduced in Colon Tissues From Patients With Inflammatory Bowel Diseases, Regulates Energy Balance in Intestinal Epithelial Cells, Epithelial Integrity, and Barrier Function.Gastroenterology · 2020Article
- Creatine metabolism: energy homeostasis, immunity and cancer biology.Nature reviews. Endocrinology · 2020Review
- The clearance of dead cells by efferocytosis.Nature reviews. Molecular cell biology · 2020Review
- The bioenergetics of neuronal morphogenesis and regeneration: Frontiers beyond the mitochondrion.Developmental neurobiology · 2020Review
- Analyzing the impact of Mycobacterium tuberculosis infection on primary human macrophages by combined exploratory and targeted metabolomics.Scientific reports · 2020Article
- Pneumonia recovery reprograms the alveolar macrophage pool.JCI insight · 2020Article
- Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.Cell reports · 2019Article
- SWAP70 is a universal GEF-like adaptor for tethering actin to phagosomes.Small GTPases · 2019Article
- Cell-in-cell phenomena in cancer.Nature reviews. Cancer · 2018Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis requires locally coordinated cytoskeletal rearrangements driven by actin polymerization and myosin motor activity. How this actomyosin dynamics is dependent upon systems that provide access to ATP at phagosome microdomains has not been determined. We analyzed the role of brain-type creatine kinase (CK-B), an enzyme involved in high-energy phosphoryl transfer. We demonstrate that endogenous CK-B in macrophages is mobilized from the cytosolic pool and coaccumulates with F-actin at nascent phagosomes. Live cell imaging with XFP-tagged CK-B and beta-actin revealed the transient and specific nature of this partitioning process. Overexpression of a catalytic dead CK-B or CK-specific cyclocreatine inhibition caused a significant reduction of actin accumulation in the phagocytic cup area, and reduced complement receptor-mediated, but not Fc-gammaR-mediated, ingestion capacity of macrophages. Finally, we found that inhibition of CK-B affected phagocytosis already at the stage of particle adhesion, most likely via effects on actin polymerization behavior. We propose that CK-B activity in macrophages contributes to complement-induced F-actin assembly events in early phagocytosis by providing local ATP supply.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.