ArticlePloS one2008
The anti-proliferative effects of the CHFR depend on the forkhead associated domain, but not E3 ligase activity mediated by ring finger domain.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- The cross talk of ubiquitination and chemotherapy tolerance in colorectal cancer.Journal of cancer research and clinical oncology · 2024Review
- Human-Derived Corneal Epithelial Cells Expressing Cell Cycle Regulators as a New Resource forFrontiers in genetics · 2019Article
- Article
- miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway.BMC cancer · 2017Article
- Ubiquitin proteasome system research in gastrointestinal cancer.World journal of gastrointestinal oncology · 2016Review
- Article
- Emerging evidence for CHFR as a cancer biomarker: from tumor biology to precision medicine.Cancer metastasis reviews · 2014Review
- Acute sensitization of colon cancer cells to inflammatory cytokines by prophase arrest.Biochemical pharmacology · 2012Article
- CHFR: a key checkpoint component implicated in a wide range of cancers.Cellular and molecular life sciences : CMLS · 2012Review
- The Stil protein regulates centrosome integrity and mitosis through suppression of Chfr.Journal of cell science · 2011Article
- Deficiencies in Chfr and Mlh1 synergistically enhance tumor susceptibility in mice.The Journal of clinical investigation · 2009Article
- CHFR, a potential tumor suppressor, downregulates interleukin-8 through the inhibition of NF-kappaB.Oncogene · 2009Article
- Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.World journal of gastroenterology · 2008Article
- CHFR: A Novel Mitotic Checkpoint Protein and Regulator of Tumorigenesis.Translational oncology · 2008Article
- Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.Neoplasia (New York, N.Y.) · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CHFR protein comprises fork head associated- (FHA) and RING-finger (RF) domain and is frequently downregulated in human colon and gastric cancers up to 50%. The loss of CHFR mRNA expression is a consequence of promoter methylation, suggesting a tumor suppressor role for this gene in gastrointestinal carcinogenesis. In terms of the biological functions of CHFR, it has been shown to activate cell cycle checkpoint when cells are treated with microtubule depolymerizing agents. Furthermore, CHFR was reported to have E3 ligase activity and promote ubiquitination and degradation of oncogenic proteins such as Aurora A and polo-like kinase 1. However, molecular pathways involved in the tumor suppressive function of CHFR are not yet clear since the two established roles of this protein are likely to inhibit cell growth. In this study, we have identified that the FHA domain of CHFR protein is critical for growth suppressive properties, whereas the RF and cysteine rich domains (Cys) are not required for this function. In contrast, the RF and Cys domains are essential for E3 ligase activity of CHFR. By the use of a cell cycle checkpoint assay, we also confirmed that the FHA domain of CHFR plays an important role in initiating a cell cycle arrest at G2/M, indicating a functional link exists between the anti-proliferative effects and checkpoint function of this tumor suppressor protein via this domain. Collectively, our data show that the checkpoint function of the FHA domain of CHFR is a core component of anti-proliferative properties against the gastrointestinal carcinogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.