ArticleJournal of cell science2008
Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- Phosphoinositide signaling at the cytoskeleton in the regulation of cell dynamics.Cell death & disease · 2025Review
- Genomics of a sexually selected sperm ornament and female preference in Drosophila.Nature ecology & evolution · 2025Article
- Cholesterol and Phosphoinositides in Cilia Biology.Advances in experimental medicine and biology · 2023Review
- Article
- Pharmacological cholesterol depletion disturbs ciliogenesis and ciliary function in developing zebrafish.Communications biology · 2019Article
- Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization.Cell division · 2019Article
- Regulation of PI4P levels by PI4KIIIα during G-protein-coupled PLC signaling inJournal of cell science · 2018Article
- γ-TuRC Heterogeneity Revealed by Analysis of Mozart1.Current biology : CB · 2018Article
- The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developingDevelopment (Cambridge, England) · 2018Article
- Layer-specific lipid signatures in the human subventricular zone demonstrated by imaging mass spectrometry.Scientific reports · 2018Article
- Novel Approach to the Development of Functional Goat's Milk-Based Beverages Using Medicinal Plant Extracts in Combination with High Intensity Ultrasound Treatment.Food technology and biotechnology · 2017Article
- INPP5E regulates phosphoinositide-dependent cilia transition zone function.The Journal of cell biology · 2017Article
- Transition zone assembly and its contribution to axoneme formation in Drosophila male germ cells.The Journal of cell biology · 2016Article
- Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.Nature communications · 2016Article
- Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.Open biology · 2016Article
- Review
- A migrating ciliary gate compartmentalizes the site of axoneme assembly in Drosophila spermatids.Current biology : CB · 2014Article
- PI(4,5)P2 regulates myoblast fusion through Arp2/3 regulator localization at the fusion site.Development (Cambridge, England) · 2014Article
- Depletion of PtdIns(4,5)P₂ underlies retinal degeneration in Drosophila trp mutants.Journal of cell science · 2013Article
- Unique properties of Drosophila spermatocyte primary cilia.Biology open · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Axonemes are microtubule-based organelles of crucial importance in the structure and function of eukaryotic cilia and flagella. Despite great progress in understanding how axonemes are assembled, the signals that initiate axoneme outgrowth remain unknown. Here, we identified phosphatidylinositol phosphates (phosphoinositides) as key regulators of early stages of axoneme outgrowth in Drosophila melanogaster spermatogenesis. In a study of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] function in developing Drosophila male germ cells, we depleted PtdIns(4,5)P2 by expression of a potent phosphoinositide phosphatase. Phosphatase expression dramatically inhibited sperm tail formation and perturbed microtubule organization in a manner reversible by co-expression of a PtdIns 4-phosphate 5-kinase. Depletion of PtdIns(4,5)P2 caused increased levels of basal body gamma-tubulin and altered the distribution of proteins known to be required for axoneme assembly. Examination of PtdIns(4,5)P2-depleted spermatids by transmission electron microscopy revealed defects in basal body docking to the nuclear envelope, and in axoneme architecture and integrity of the developing flagellar axoneme and axial sheath. Our results provide the first evidence that phosphoinositides act at several steps during flagellar biogenesis, coordinately regulating microtubule and membrane organization. They further suggest that phosphoinositides play evolutionarily conserved roles in flagella and cilia, across phyla and in structurally diverse cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.