ArticleMicrobial cell factories2008
Expression in Escherichia coli, purification, refolding and antifungal activity of an osmotin from Solanum nigrum.
Article in Microbial cell factories, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections.Synthetic and systems biotechnology · 2024Article
- Tomato-Thaumatin-like Protein GenesGenes · 2023Article
- The potential of plant proteins as antifungal agents for agricultural applications.Synthetic and systems biotechnology · 2022Review
- Antimicrobial Peptides from Plants: A cDNA-Library Based Isolation, Purification, Characterization Approach and Elucidating Their Modes of Action.International journal of molecular sciences · 2021Review
- ROS Dependent Antifungal and Anticancer Modulations ofMolecules (Basel, Switzerland) · 2021Article
- The Osmotin-Like Protein GeneInternational journal of molecular sciences · 2020Article
- Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.Transgenic research · 2018Article
- Molecular Cloning and Expression of Osmotin in a Baculovirus-Insect System: Purified Osmotin Mitigates Amyloid-beta Deposition in Neuronal Cells.Scientific reports · 2017Article
- Cloning, characterization, and bacterial over-expression of an osmotin-like protein gene from Solanum nigrum L. with antifungal activity against three necrotrophic fungi.Molecular biotechnology · 2015Article
- The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca(2+) and Mg(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa.BMC plant biology · 2014Article
- N-terminal in coat protein of Garlic virus X is indispensible for its serological detection.Virus genes · 2014Article
- Tandem combination of Trigonella foenum-graecum defensin (Tfgd2) and Raphanus sativus antifungal protein (RsAFP2) generates a more potent antifungal protein.Functional & integrative genomics · 2013Article
- Increased resistance to late leaf spot disease in transgenic peanut using a combination of PR genes.Functional & integrative genomics · 2012Article
- High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin.Transgenic research · 2011Article
- Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar.BMC plant biology · 2011Article
- Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl.Proteome science · 2010Article
- Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.).Protoplasma · 2010Article
- The superfamily of thaumatin-like proteins: its origin, evolution, and expression towards biological function.Plant cell reports · 2010Review
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHeterologous protein expression in microorganisms may contribute to identify and demonstrate antifungal activity of novel proteins. The Solanum nigrum osmotin-like protein (SnOLP) gene encodes a member of pathogenesis-related (PR) proteins, from the PR-5 sub-group, the last comprising several proteins with different functions, including antifungal activity. Based on deduced amino acid sequence of SnOLP, computer modeling produced a tertiary structure which is indicative of antifungal activity.
resultsTo validate the potential antifungal activity of SnOLP, a hexahistidine-tagged mature SnOLP form was overexpressed in Escherichia coli M15 strain carried out by a pQE30 vector construction. The urea solubilized His6-tagged mature SnOLP protein was affinity-purified by immobilized-metal (Ni2+) affinity column chromatography. As SnOLP requires the correct formation of eight disulfide bonds, not correctly formed in bacterial cells, we adapted an in vitro method to refold the E. coli expressed SnOLP by using reduced:oxidized gluthatione redox buffer. This method generated biologically active conformations of the recombinant mature SnOLP, which exerted antifungal action towards plant pathogenic fungi (Fusarium solani f. sp.glycines, Colletotrichum spp., Macrophomina phaseolina) and oomycete (Phytophthora nicotiana var. parasitica) under in vitro conditions.
conclusionSince SnOLP displays activity against economically important plant pathogenic fungi and oomycete, it represents a novel PR-5 protein with promising utility for biotechnological applications.
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