ArticleJournal of health, population, and nutrition2007
Vibrio cholerae in the environment: a simple method for reliable identification of the species.
Article in Journal of health, population, and nutrition, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Vibrio cholerae-An emerging pathogen in Austrian bathing waters?Wiener klinische Wochenschrift · 2023Review
- Review of Rapid Diagnostic Methods forThe East African health research journal · 2023Article
- A Fatal Case of Bacteremia Caused byInfection & chemotherapy · 2021Article
- Simultaneous Quantification ofPathogens (Basel, Switzerland) · 2020Article
- Geographical distribution and antibiotics susceptibility patterns of toxigenic Vibrio cholerae isolates from Kisumu County, Kenya.African journal of primary health care & family medicine · 2020Article
- Vibrio cholerae non-O1 - the first reported case of keratitis in a healthy patient.BMC infectious diseases · 2019Article
- Application of three different methods to determine the prevalence, the abundance and the environmental drivers of culturable Vibrio cholerae in fresh and brackish bathing waters.Journal of applied microbiology · 2018Article
- Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay.Scientific reports · 2018Article
- Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139Frontiers in microbiology · 2017Article
- Antimicrobial Susceptibility of Autochthonous AquaticFrontiers in microbiology · 2016Article
- Dynamics of Vibrio cholerae abundance in Austrian saline lakes, assessed with quantitative solid-phase cytometry.Environmental microbiology · 2015Article
- A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.Applied and environmental microbiology · 2015Article
- No Evidence of Significant Levels of Toxigenic V. cholerae O1 in the Haitian Aquatic Environment During the 2012 Rainy Season.PLoS currents · 2013Article
- Rapid and sensitive quantification of Vibrio cholerae and Vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple screening and identification protocol was assessed for the efficient distinction of colonies of Vibrio cholerae species from others obtained on thiosulphate citrate bile salts sucrose agar after isolation from different environmental specimens. It was demonstrated here that the yellow colonies (sucrose-fermenting), which are able to grow on nutrient agar without added NaCl and which present a positive oxidase reaction, can be confidently considered as presumptive V. cholerae. Confirmation of the identification was carried out using the API 20E microtest and by species-specific ompW-based polymerase chain reaction: 809 of 925 isolates obtained by this screening procedure were identified as V. cholerae by API 20E and confirmed by PCR. The results showed that the direct use of the PCR-based method for the definite identification of the screened colonies gave better results than the API 20E method: of a selection of 100 isolates presumptively identified as V. cholerae according to the proposed screening procedure, all gave a positive result with PCR but only 94 were confirmed by API 20E. This protocol provides reliable identification of V. cholerae species and is adapted to the capabilities of routine clinical, food-testing and environmental microbiology laboratories.
Indexed as
Identifiers
18330064PMC2754027W1895903829What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.