ArticleYeast (Chichester, England)2008
Direct selection of Pichia pastoris expression strains using new G418 resistance vectors.
Article in Yeast (Chichester, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione.Microbial cell factories · 2025Article
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.FEMS yeast research · 2025Review
- Optimal fermentation conditions for growth and recombinant protein production inCurrent research in food science · 2024Article
- A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting inSynthetic and systems biotechnology · 2023Article
- High-level expression of leghemoglobin inFrontiers in bioengineering and biotechnology · 2023Article
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- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Role ofApplied and environmental microbiology · 2019Article
- A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization.Protein expression and purification · 2018Article
- Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker.Microbial cell factories · 2017Article
- Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement inEnzyme research · 2017Article
- Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications.World journal of microbiology & biotechnology · 2017Review
- Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris.Microbial cell factories · 2016Article
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- Screening of multi-copy mannanase recombinants of Pichia pastoris based on colony size.World journal of microbiology & biotechnology · 2014Article
- Can too many copies spoil the broth?Microbial cell factories · 2013Review
- Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins.Biotechnology letters · 2013Article
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Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants. In this report we describe new expression vectors that can be used to select directly for P. pastoris transformants using G418 resistance conferred by a modified Tn903kan(r) gene. Compared to other dominant markers, this system is more economical and offers a higher transformation efficiency, due to the small sizes of the cloning vectors, pKAN B and pKANalpha B (GenBank Accession Nos EU285585 and EU285586, respectively). Additionally, multicopy transformants can be generated using these new vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.