ArticleProceedings of the National Academy of Sciences of the United States of America1991
Identification and molecular cloning of a soluble human granulocyte-macrophage colony-stimulating factor receptor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 104 citations in OpenAlex.
- Cytokine receptor splice variants in hematologic diseases.Cytokine · 2020Review
- Granulocyte-macrophage colony-stimulating factor: not just another haematopoietic growth factor.Medical oncology (Northwood, London, England) · 2014Review
- A cell free assay system estimating the neutralizing capacity of GM-CSF antibody using recombinant soluble GM-CSF receptor.Journal of visualized experiments : JoVE · 2011Article
- GM-CSF: a role in immune and inflammatory reactions in the intestine.Expert review of gastroenterology & hepatology · 2010Article
- Characterisation of the expression and function of the GM-CSF receptor alpha-chain in mice.European journal of immunology · 2007Article
- The Human Pseudoautosomal Region (PAR): Origin, Function and Future.Current genomics · 2007Article
- Differential use of signal peptides and membrane domains is a common occurrence in the protein output of transcriptional units.PLoS genetics · 2006Article
- Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.Journal of virology · 2000Article
- Alternatively spliced forms in the cytoplasmic domain of the human growth hormone (GH) receptor regulate its ability to generate a soluble GH-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Highly metastatic 13762NF rat mammary adenocarcinoma cell clones stimulate bone marrow by secretion of granulocyte-macrophage colony-stimulating factor/interleukin-3 activity.The American journal of pathology · 1995Article
- PU.1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating factor receptor alpha gene.Molecular and cellular biology · 1995Article
- Membrane-associated and soluble granulocyte/macrophage-colony-stimulating factor receptor alpha subunits are independently regulated in HL-60 cells.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- High affinity immunoreactive FGF receptors in the extracellular matrix of vascular endothelial cells--implications for the modulation of FGF-2.The Journal of cell biology · 1995Article
- Identification of soluble forms of the fibroblast growth factor receptor in blood.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Soluble receptors for cytokines and growth factors: generation and biological function.The Biochemical journal · 1994Review
- The alpha subunit of the human granulocyte-macrophage colony-stimulating factor receptor signals for glucose transport via a phosphorylation-independent pathway.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Alternative promoter usage of the Fos-responsive gene Fit-1 generates mRNA isoforms coding for either secreted or membrane-bound proteins related to the IL-1 receptor.The EMBO journal · 1994Article
- Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal.The EMBO journal · 1993Article
- Differently spliced cDNAs of human leukocyte tyrosine kinase receptor tyrosine kinase predict receptor proteins with and without a tyrosine kinase domain and a soluble receptor protein.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays an important role in hematopoiesis and host defense via interaction with specific cell-surface receptors in target tissues. We identified a truncated, soluble form of the low-affinity GM-CSF receptor (GMR) in chorio-carcinoma cells. Low-affinity GMR cDNAs encoding both the membrane-bound and soluble receptors were obtained by PCR using primers corresponding to the published sequence. Clones encoding the soluble receptor were identical in sequence to the membrane-bound form but contained a 97-nucleotide internal deletion. The amino acid sequence of this deleted cDNA predicts a protein that lacks the 84 C-terminal amino acids of the membrane-bound receptor, including the transmembrane and cytoplasmic domains, and contains 16 different amino acids at its C terminus. Expression of the soluble GMR cDNA in murine psi-AM cells as well as GM-CSF-dependent myeloid 32Dc13 cells produced a secreted protein that retained its capacity to bind GM-CSF in solution. RNase protection analysis indicates that this variant cDNA is derived from a naturally occurring mRNA. Soluble receptors have been identified for several other hematopoietin receptors and may be a general feature of this class. The striking similarity between the soluble form of the GMR and other hematopoietin receptors suggests that soluble binding proteins may play an important role in regulating the broad spectrum of biological responses mediated by these cytokines.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.