ArticleBiophysical journal2008
Controlled aggregation of ferritin to modulate MRI relaxivity.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- MatchingFrontiers in neuroanatomy · 2019Pooled it
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- Coherent X-rays reveal anomalous molecular diffusion and cage effects in crowded protein solutions.Nature communications · 2025Article
- Non-invasive assessment of normal and impaired iron homeostasis in the brain.Nature communications · 2023Article
- Engineering Self-Assembling Protein Nanoparticles for Therapeutic Delivery.Bioconjugate chemistry · 2022Review
- Conservative and Atypical Ferritins of Sponges.International journal of molecular sciences · 2021Article
- Longitudinal and Transverse Relaxivity Analysis of Native Ferritin and Magnetoferritin at 7 T MRI.International journal of molecular sciences · 2021Article
- Surface radio-mineralisation mediates chelate-free radiolabelling of iron oxide nanoparticles.Chemical science · 2019Article
- Structure-Relaxivity Relationships of Magnetic Nanoparticles for Magnetic Resonance Imaging.Advanced materials (Deerfield Beach, Fla.) · 2019Review
- Molecular fMRI.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Dynamic contrast-enhanced quantitative susceptibility mapping with ultrashort echo time MRI for evaluating renal function.American journal of physiology. Renal physiology · 2016Article
- Engineering intracellular biomineralization and biosensing by a magnetic protein.Nature communications · 2015Article
- Cationized ferritin as a magnetic resonance imaging probe to detect microstructural changes in a rat model of non-alcoholic steatohepatitis.Magnetic resonance in medicine · 2013Article
- MRI reporter genes: applications for imaging of cell survival, proliferation, migration and differentiation.NMR in biomedicine · 2013Review
- Metalloprotein-based MRI probes.FEBS letters · 2013Review
- Magnetic Nanoclusters with Hydrophilic Spacing for Dual Drug Delivery and Sensitive Magnetic Resonance Imaging.Journal of materials chemistry. B · 2013Article
- Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium.PloS one · 2013Article
- Is there a path beyond BOLD? Molecular imaging of brain function.NeuroImage · 2012Review
- The ferritin superfamily: Supramolecular templates for materials synthesis.Biochimica et biophysica acta · 2010Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Ferritin is an iron storage protein expressed in varying concentrations in mammalian cells. The deposition of ferric iron in the core of ferritin makes it a magnetic resonance imaging contrast agent, and ferritin has recently been proposed as a gene expression reporter protein for magnetic resonance imaging. To date, ferritin has been overexpressed in vivo and has been coexpressed with transferrin receptor to increase iron loading in cells. However, ferritin has a relatively low T(2) relaxivity (R(2) approximately 1 mM(-1)s(-1)) at typical magnetic field strengths and so requires high levels of expression to be detected. One way to modulate the transverse relaxivity of a superparamagnetic agent is to cause it to aggregate, thereby manipulating the magnetic field gradients through which water diffuses. In this work, it is demonstrated by computer simulation and in vitro that aggregation of ferritin can alter relaxivity. The effects of aggregate size and intraaggregate perturber spacing on R(2) are studied. Computer modeling indicates that the optimal spacing of the ferritin molecules in aggregate for increasing R(2) is 100-200 nm for a typical range of water diffusion rates. Chemical cross-linking of ferritin at 12 A spacing led to a 70% increase in R(2) compared to uncross-linked ferritin controls. To modulate ferritin aggregation in a potentially biologically relevant manner, ferritin was attached to actin and polymerized in vitro. The polymerization of ferritin-F-actin caused a 20% increase in R(2) compared to unpolymerized ferritin-G-actin. The R(2)-value was increased by another 10% by spacing the ferritin farther apart on the actin filaments. The modulation of ferritin aggregation by binding to cytoskeletal elements may be a useful strategy to make a functional reporter gene for magnetic resonance imaging.
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Registered trials
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