ArticleBiophysical journal2008
Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains.Small science · 2025Article
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes.Open biology · 2023Article
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- Cell and tissue morphology determine actin-dependent nuclear migration mechanisms in neuroepithelia.The Journal of cell biology · 2019Article
- Nck deficiency is associated with delayed breast carcinoma progression and reduced metastasis.Molecular biology of the cell · 2017Article
- Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Control of polarized assembly of actin filaments in cell motility.Cellular and molecular life sciences : CMLS · 2015Review
- Emergent complexity of the cytoskeleton: from single filaments to tissue.Advances in physics · 2013Review
- Interactions of isolated C-terminal fragments of neural Wiskott-Aldrich syndrome protein (N-WASP) with actin and Arp2/3 complex.The Journal of biological chemistry · 2012Article
- Cytoskeletal modulation of lipid interactions regulates Lck kinase activity.The Journal of biological chemistry · 2012Article
- Choosing orientation: influence of cargo geometry and ActA polarization on actin comet tails.Molecular biology of the cell · 2012Article
- Actin polymerization controls the organization of WASH domains at the surface of endosomes.PloS one · 2012Article
- Actin filament attachments for sustained motility in vitro are maintained by filament bundling.PloS one · 2012Article
- Article
- Actin disassembly 'clock' and membrane tension determine cell shape and turning: a mathematical model.Journal of physics. Condensed matter : an Institute of Physics journal · 2010Article
- Force generation of curved actin gels characterized by combined AFM-epifluorescence measurements.Biophysical journal · 2010Article
- A simple method for the reconstitution of membrane proteins into giant unilamellar vesicles.The Journal of membrane biology · 2010Article
- How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach.The EMBO journal · 2010Article
- Physical mechanisms of signal integration by WASP family proteins.Annual review of biochemistry · 2010Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatially controlled assembly of actin in branched filaments generates cell protrusions or the propulsion of intracellular vesicles and pathogens. The propulsive movement of giant unilamellar vesicles (GUVs) functionalized by N-WASP (full-length or truncated) is reconstituted in a biochemically controlled medium, and analyzed using phase contrast and fluorescence microscopy to elucidate the links between membrane components and the actin cytoskeleton that determine motile behavior. Actin-based propulsion displays a continuous regime or a periodic saltatory regime. The transition between the two regimes is controlled by the concentration of Arp2/3 complex, which branches filaments by interacting with N-WASP at the liposome surface. Saltatory motion is linked to cycles in the distribution of N-WASP at the membrane between a homogeneous and a segregated state. Comparison of the changes in distribution of N-WASP, Arp2/3, and actin during propulsion demonstrates that actin filaments bind to N-WASP, and that these bonds are transitory. This interaction, mediated by Arp2/3, drives N-WASP segregation. VC-fragments of N-WASP, that interact more weakly than N-WASP with the Arp2/3 complex, segregate less than N-WASP at the rear of the GUVs. GUV propulsion is inhibited by the presence of VCA-actin covalent complex, showing that the release of actin from the nucleator is required for movement. The balance between segregation and free diffusion determines whether continuous movement can be sustained. Computed surface distributions of N-WASP, derived from a theoretical description of this segregation-diffusion mechanism, account satisfactorily for the measured density profiles of N-WASP, Arp2/3 complex, and actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.