ArticleThe Journal of biological chemistry2008
A novel GDP-dependent pyruvate kinase isozyme from Toxoplasma gondii localizes to both the apicoplast and the mitochondrion.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 52 citations in OpenAlex.
- Engineering riboflavin-overproducingSynthetic and systems biotechnology · 2026Article
- Two apicoplast dwelling glycolytic enzymes provide key substrates for metabolic pathways in the apicoplast and are critical for Toxoplasma growth.PLoS pathogens · 2022Article
- The lid domain is important, but not essential, for catalysis of Escherichia coli pyruvate kinase.European biophysics journal : EBJ · 2020Article
- Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea.Open biology · 2020Review
- The NTP generating activity of pyruvate kinase II is critical for apicoplast maintenance ineLife · 2020Article
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- An overview of structure, function, and regulation of pyruvate kinases.Protein science : a publication of the Protein Society · 2019Review
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- Elucidating the mitochondrial proteome ofeLife · 2018Article
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- Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis, a 53.1-kDa homopentamer, implicated immune protective efficacy against clonorchiasis.Parasites & vectors · 2017Article
- The role of pyruvate hub enzymes in supplying carbon precursors for fatty acid synthesis in photosynthetic microalgae.Photosynthesis research · 2015Article
- Dual Organellar Targeting of Aminoacyl-tRNA Synthetases in Diatoms and Cryptophytes.Genome biology and evolution · 2015Article
- Evidence for loss of a partial flagellar glycolytic pathway during trypanosomatid evolution.PloS one · 2014Article
- Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii.Cell host & microbe · 2012Article
- Crystal structure of Cryptosporidium parvum pyruvate kinase.PloS one · 2012Article
- Broad distribution of TPI-GAPDH fusion proteins among eukaryotes: evidence for glycolytic reactions in the mitochondrion?PloS one · 2012Article
- Article
- Rewiring and regulation of cross-compartmentalized metabolism in protists.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported a cytosolic pyruvate kinase (EC 2.7.1.40) from Toxoplasma gondii (TgPyKI) that differs from most eukaryotic pyruvate kinases in being regulated by glucose 6-phosphate rather than fructose 1,6-diphosphate. Another putative pyruvate kinase (TgPyKII) was identified from parasite genome, which exhibits 32% amino acid sequence identity to TgPyKI and retains pyruvate kinase signature motifs and amino acids essential for substrate binding and catalysis. Whereas TgPyKI is most closely related to plant/algal enzymes, phylogenetic analysis suggests a proteobacterial origin for TgPyKII. Enzymatic characterization of recombinant TgPyKII shows a high pH optimum at 8.5, and a preference for GDP as a phosphate recipient. Catalytic activity is independent of K+, and no allosteric or regulatory effects were observed in the presence of fructose 1,6-diphosphate, fructose 2,6-diphosphate, glucose 6-phosphate, ribose 5-phosphate, AMP, or ATP. Unlike TgPyKI, native TgPyKII activity was exclusively associated with the membranous fraction of a T. gondii tachyzoite lysate. TgPyKII possesses a long N-terminal extension containing five putative start codons before the conserved region and localizes to both apicoplast and mitochondrion by immunofluorescence assay using native antibody and fluorescent protein fusion to the N-terminal extension. Further deletional and site-directed mutagenesis suggests that a translation product from 1st Met is responsible for the localization to the apicoplast, whereas one from 3rd Met is for the mitochondrion. This is the first study of a potential mitochondrial pyruvate kinase in any system.
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