ArticleMolecular and biochemical parasitology2008
Characterization of the mitochondrial inner membrane protein translocator Tim17 from Trypanosoma brucei.
Article in Molecular and biochemical parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 57 citations in OpenAlex.
- TbTim20 facilitates protein import at a low membrane potential in trypanosomes lacking the mitochondrial genome.The FEBS journal · 2026Article
- Article
- Is the mitochondrion a promising drug target in trypanosomatids?Memorias do Instituto Oswaldo Cruz · 2022Article
- Novel IM-associated protein Tim54 plays a role in the mitochondrial import of internal signal-containing proteins in Trypanosoma brucei.Biology of the cell · 2021Article
- Tim17 Updates: A Comprehensive Review of an Ancient Mitochondrial Protein Translocator.Biomolecules · 2020Review
- tRNA import across the mitochondrial inner membrane in T. brucei requires TIM subunits but is independent of protein import.Nucleic acids research · 2020Article
- Article
- The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes.Redox biology · 2020Article
- Independent accretion of TIM22 complex subunits in the animal and fungal lineages.F1000Research · 2020Article
- Quantitative phosphoproteome and proteome analyses emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis.Cell stress & chaperones · 2019Article
- Article
- A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes.Genome biology and evolution · 2018Article
- Divergent Small Tim Homologues Are Associated with TbTim17 and Critical for the Biogenesis of TbTim17 Protein Complexes inmSphere · 2018Article
- Cultured bloodstream Trypanosoma brucei adapt to life without mitochondrial translation release factor 1.Scientific reports · 2018Article
- The divergent N-terminal domain of Tim17 is critical for its assembly in the TIM complex in Trypanosoma brucei.Molecular and biochemical parasitology · 2017Article
- Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei.PLoS pathogens · 2017Article
- Origin and Evolutionary Alteration of the Mitochondrial Import System in Eukaryotic Lineages.Molecular biology and evolution · 2017Article
- The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins.Nature communications · 2016Article
- Evolution of the Tim17 protein family.Biology direct · 2016Article
- The Import of Proteins into the Mitochondrion of Toxoplasma gondii.The Journal of biological chemistry · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Mitochondrial protein translocation machinery in the kinetoplastid parasites, like Trypanosoma brucei, has been characterized poorly. In T. brucei genome database, one homolog for a protein translocator of mitochondrial inner membrane (Tim) has been found, which is closely related to Tim17 from other species. The T. brucei Tim17 (TbTim17) has a molecular mass 16.2kDa and it possesses four characteristic transmembrane domains. The protein is localized in the mitochondrial inner membrane. The level of TbTim17 protein is 6-7-fold higher in the procyclic form that has a fully active mitochondrion, than in the mammalian bloodstream form of T. brucei, where many of the mitochondrial activities are suppressed. Knockdown of TbTim17 expression by RNAi caused a cessation of cell growth in the procyclic form and reduced growth rate in the bloodstream form. Depletion of TbTim17 decreased mitochondrial membrane potential more in the procyclic than bloodstream form. However, TbTim17 knockdown reduced the expression level of several nuclear encoded mitochondrial proteins in both the forms. Furthermore, import of presequence containing nuclear encoded mitochondrial proteins was significantly reduced in TbTim17 depleted mitochondria of the procyclic as well as the bloodstream form, confirming that TbTim17 is critical for mitochondrial protein import in both developmental forms. Together, these show that TbTim17 is the translocator of nuclear encoded mitochondrial proteins and its expression is regulated according to mitochondrial activities in T. brucei.
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