ArticleBiochimica et biophysica acta2008
Zinc mesoporphyrin induces rapid and marked degradation of the transcription factor Bach1 and up-regulates HO-1.
Article in Biochimica et biophysica acta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Enhancement of Rotenone Cytotoxicity in the Presence of Bach1 Inhibitors.Doklady. Biochemistry and biophysics · 2026Article
- The Multifaceted Roles of BACH1 in Disease: Implications for Biological Functions and Therapeutic Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Antioxidant and oxidative enzymes, genetic variants, and cofactors as prognostic biomarkers of COVID-19 severity and mortality: a systematic review.Frontiers in molecular biosciences · 2025Review
- Update on heme biosynthesis, tissue-specific regulation, heme transport, relation to iron metabolism and cellular energy.Liver international : official journal of the International Association for the Study of the Liver · 2024Review
- Interactions between zinc and NRF2 in vascular redox signalling.Biochemical Society transactions · 2024Review
- A Stochastic Binary Model for the Regulation of Gene Expression to Investigate Responses to Gene Therapy.Cancers · 2022Article
- Review
- Article
- Clinically important features of porphyrin and heme metabolism and the porphyrias.Metabolites · 2014Review
- Characterization of docosahexaenoic acid (DHA)-induced heme oxygenase-1 (HO-1) expression in human cancer cells: the importance of enhanced BTB and CNC homology 1 (Bach1) degradation.The Journal of nutritional biochemistry · 2014Article
- Role of H2O2 in the oxidative effects of zinc exposure in human airway epithelial cells.Redox biology · 2014Article
- Heme status affects human hepatic messenger RNA and microRNA expression.World journal of gastroenterology · 2013Article
- Metalloporphyrins - an update.Frontiers in pharmacology · 2012Article
- Heme and HO-1 Inhibition of HCV, HBV, and HIV.Frontiers in pharmacology · 2012Article
- Effects of zinc deuteroporphyrin bis glycol on newborn mice after heme loading.Pediatric research · 2011Article
- Zinc mesoporphyrin induces rapid proteasomal degradation of hepatitis C nonstructural 5A protein in human hepatoma cells.Gastroenterology · 2010Article
- MicroRNA-196 represses Bach1 protein and hepatitis C virus gene expression in human hepatoma cells expressing hepatitis C viral proteins.Hepatology (Baltimore, Md.) · 2010Article
- Zinc protoporphyrin regulates cyclin D1 expression independent of heme oxygenase inhibition.The Journal of biological chemistry · 2009Article
- Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells.World journal of gastroenterology · 2009Article
- Role of haem oxygenase-1 in microbial host defence.Cellular microbiology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Heme oxygenase 1 (HO-1) is the first and rate-controlling enzyme in heme degradation. Bach1 is a mammalian transcriptional repressor of HO-1. To understand how zinc mesoporphyrin (ZnMP) induces the expression of HO-1, we investigated the effects of ZnMP on Bach1 mRNA and protein levels in human hepatoma Huh-7 cells by quantitative RT-PCR and Western blots. We found that ZnMP markedly up-regulated HO-1 mRNA and protein levels, and rapidly and significantly decreased Bach1 protein levels by increasing degradation of Bach1 protein [half life (t(1/2)) from 19 h to 45 min], whereas ZnMP did not influence Bach1 mRNA levels. The proteasome inhibitors, epoxomicin and MG132, significantly inhibited degradation of Bach1 by ZnMP in a dose-dependent fashion, indicating that the degradation of Bach1 by ZnMP is proteasome-dependent. Purified Bach1 C-terminal fragment bound heme, but there was no evidence for binding of ZnMP to the heme-binding region of Bach1. In conclusion, ZnMP produces profound post-transcriptional down-regulation of Bach1 protein levels and transcriptional up-regulation of HO-1. Our results indicate that ZnMP up-regulates HO-1 gene expression by markedly increasing Bach1 protein degradation in a proteasome-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.