ArticleEmerging infectious diseases2008
Discovering and differentiating new and emerging clonal populations of Chlamydia trachomatis with a novel shotgun cell culture harvest assay.
Article in Emerging infectious diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Whole-genome automated assembly pipeline forNAR genomics and bioinformatics · 2025Article
- Genomic insights into local-scale evolution of ocularMicrobial genomics · 2024Article
- Patterns of within-host spread ofbioRxiv : the preprint server for biology · 2023Article
- Patterns of within-host spread ofFrontiers in microbiology · 2023Article
- Comparison of genovars and Chlamydia trachomatis infection loads in ocular samples from children in two distinct cohorts in Sudan and Morocco.PLoS neglected tropical diseases · 2021Article
- Prevalence ofInternational journal of environmental research and public health · 2019Article
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- Rapid and sensitive detection of Chlamydia trachomatis sexually transmitted infections in resource-constrained settings in Thailand at the point-of-care.PLoS neglected tropical diseases · 2018Article
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- Rapid detection and strain typing of Chlamydia trachomatis using a highly multiplexed microfluidic PCR assay.PloS one · 2017Article
- Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.Microbiology and molecular biology reviews : MMBR · 2016Review
- Population genomics of Chlamydia trachomatis: insights on drift, selection, recombination, and population structure.Molecular biology and evolution · 2012Article
- Engineered phage-based therapeutic materials inhibit Chlamydia trachomatis intracellular infection.Biomaterials · 2012Article
- In vitro recombinants of antibiotic-resistant Chlamydia trachomatis strains have statistically more breakpoints than clinical recombinants for the same sequenced loci and exhibit selection at unexpected loci.Journal of bacteriology · 2012Article
- Article
- Mud crab susceptibility to disease from white spot syndrome virus is species-dependent.BMC research notes · 2010Article
- Predicting phenotype and emerging strains among Chlamydia trachomatis infections.Emerging infectious diseases · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia trachomatis is the leading cause of preventable blindness and bacterial sexually transmitted diseases worldwide. Plaque assays have been used to clonally segregate laboratory-adapted C. trachomatis strains from mixed infections, but no assays have been reported to segregate clones from recent clinical samples. We developed a novel shotgun cell culture harvest assay for this purpose because we found that recent clinical samples do not form plaques. Clones were strain-typed by using outer membrane protein A and 16S rRNA sequences. Surprisingly, ocular trachoma reference strain A/SA-1 contained clones of Chlamydophila abortus. C. abortus primarily infects ruminants and pigs and has never been identified in populations where trachoma is endemic. Three clonal variants of reference strain Ba/Apache-2 were also identified. Our findings reflect the importance of clonal isolation in identifying constituents of mixed infections containing new or emerging strains and of viable clones for research to more fully understand the dynamics of in vivo strain-mixing, evolution, and disease pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.