ArticleBMC molecular biology2008
Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 221 citations in OpenAlex.
- Screening of Stable Reference Genes inVeterinary sciences · 2026Article
- Comparative evaluation of four reference genes for qRT-PCR assays in snow trout (Schizothorax richardsonii) under multiple experimental conditions.Scientific reports · 2026Article
- Impact of different levels of handling on Solea senegalensis culture: effects on growth and molecular markers of stress.Fish physiology and biochemistry · 2024Article
- Systematic selection of suitable reference genes for quantitative real-time PCR normalization studies of gene expression in Lutjanus erythropterus.Scientific reports · 2024Article
- Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole.Animals : an open access journal from MDPI · 2023Article
- Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions.Fish physiology and biochemistry · 2021Article
- Dietary Natural Plant Extracts Can Promote Growth and Modulate Oxidative Status of Senegalese Sole Postlarvae under Standard/Challenge Conditions.Animals : an open access journal from MDPI · 2021Article
- A Tentative Study of the Effects of Heat-Inactivation of the Probiotic StrainMicroorganisms · 2021Article
- Cold Adaptation in Antarctic Notothenioids: Comparative Transcriptomics Reveals Novel Insights in the Peculiar Role of Gills and Highlights Signatures of Cobalamin Deficiency.International journal of molecular sciences · 2021Article
- New Insights on Vitamin K Metabolism in Senegalese sole (International journal of molecular sciences · 2020Article
- Ontogenetic expression rhythms of visual opsins in senegalese sole are modulated by photoperiod and light spectrum.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Article
- Dietary Antioxidant Supplementation Promotes Growth in Senegalese Sole Postlarvae.Frontiers in physiology · 2020Article
- Assessment of Growth, Lipid Metabolism and Gene Expression Responses in Senegalese Sole Larvae Fed With Low Dietary Phospholipid Levels.Frontiers in physiology · 2020Article
- Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.PloS one · 2020Article
- Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach.Scientific reports · 2019Article
- The organophosphate pesticide -OP- malathion inducing thyroidal disruptions and failures in the metamorphosis of the Senegalese sole, Solea senegalensis.BMC veterinary research · 2019Article
- Genomic and phylogenetic analysis of choriolysins, and biological activity of hatching liquid in the flatfish Senegalese sole.PloS one · 2019Article
- Evaluation of Reference Genes to Analyze Gene Expression in SilversideFrontiers in genetics · 2018Article
- Identification of housekeeping genes as references for quantitative real-time RT-PCR analysis in Misgurnus anguillicaudatus.Journal of genetics · 2017Article
- Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis.BMC developmental biology · 2017Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFlatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using quantitative real-time PCR (qRT-PCR) technique. Therefore, adequate reference genes for accurate normalization are required.
resultsThe stability of 12 potential reference genes was examined during larval development in Senegalese sole (Solea senegalensis) and Atlantic halibut (Hippoglossus hippoglossus) to determine the most suitable genes for qRT-PCR analysis. Transcription levels of genes encoding beta-Actin (ACTB), glyceraldehyde-3P-dehydrogenase (GAPDH), annexin A2 (ANXA2), glutathione S-transferase (GST), ornithine decarboxylase (ODC), hypoxanthine phosphoribosyltransferase (HPRT1), ubiquitin (UBQ), elongation factor 1 alpha (eEF1A1), 18S ribosomal RNA, and the ribosomal proteins S4 (RPS4) and L13a (RPL13a) were quantitated. Two paralogous genes for ACTB were analyzed in each of both flatfish species. In addition, two paralogous genes for GAPDH were studied in Senegalese sole. RPL13a represented non-orthologous genes between both flatfish species. GeNorm and NormFinder analyses for expression stability revealed RPS4, UBQ and eEF1A1 as the most stable genes in Senegalese sole, Atlantic halibut and in a combined analysis. In all cases, paralogous genes exhibited differences in expression stability.
conclusionThis work suggests RPS4, UBQ, and eEF1A1 genes as useful reference genes for accurate normalization in qRT-PCR studies in Senegalese sole and Atlantic halibut larvae. The congruent results between both species in spite of the drastic differences in larval development suggest that selected housekeeping genes (HKGs) could be useful in other flatfish species. However, the finding of paralogous gene copies differentially expressed during development in some HKGs underscores the necessity to identify orthologous genes.
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