ArticleJournal of fluorescence2008
Rapid frequency-domain FLIM spinning disk confocal microscope: lifetime resolution, image improvement and wavelet analysis.
Article in Journal of fluorescence, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Measuring Molecular Interactions with Subcellular Resolution: Single-Cell FRET Using the Quantitative Three-Filterset (Intensity-Based) Approach.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Optimal Wavelet Selection for Signal Denoising.IEEE access : practical innovations, open solutions · 2024Article
- Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis.Nature communications · 2022Article
- High-speed multifocal array scanning using refractive window tilting.Biomedical optics express · 2015Article
- Lanthanide-based imaging of protein-protein interactions in live cells.Inorganic chemistry · 2014Review
- Single-cell imaging of mechanotransduction in endothelial cells.Progress in molecular biology and translational science · 2014Review
- Article
- Förster resonance energy transfer microscopy and spectroscopy for localizing protein-protein interactions in living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Review
- FRET imaging of calcium signaling in live cells in the microenvironment.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Article
- Monitoring biosensor activity in living cells with fluorescence lifetime imaging microscopy.International journal of molecular sciences · 2012Review
- Fluorescence lifetime imaging microscopy of intracellular glucose dynamics.Journal of diabetes science and technology · 2012Article
- Red-shifted fluorescent proteins monitor enzymatic activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM).Journal of microscopy · 2012Article
- Total variation versus wavelet-based methods for image denoising in fluorescence lifetime imaging microscopy.Journal of biophotonics · 2012Article
- Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Review
- Methodological considerations for global analysis of cellular FLIM/FRET measurements.Journal of biomedical optics · 2012Article
- Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy.Methods in enzymology · 2012Review
- Phasor imaging with a widefield photon-counting detector.Journal of biomedical optics · 2012Article
- Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy.Nature protocols · 2011Article
- Non fitting based FRET-FLIM analysis approaches applied to quantify protein-protein interactions in live cells.Biophysical reviews · 2011Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A spinning disk confocal attachment is added to a full-field real-time frequency-domain fluorescence lifetime-resolved imaging microscope (FLIM). This provides confocal 3-D imaging while retaining all the characteristics of the normal 2-D FLIM. The spinning disk arrangement allows us to retain the speed of the normal 2-D full field FLIM while gaining true 3-D resolution. We also introduce the use of wavelet image transformations into the FLIM analysis. Wavelets prove useful for selecting objects according to their morphology, denoising and background subtraction. The performance of the instrument and the analysis routines are tested with quantitative physical samples and examples are presented with complex biological samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.