ArticleDiabetologia2008
Reduced EDHF responses and connexin activity in mesenteric arteries from the insulin-resistant obese Zucker rat.
Article in Diabetologia, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Transcriptional analysis reveals that the intracellular lipid accumulation impairs gene expression profiles involved in insulin response-associated cardiac functionality.Scientific reports · 2023Article
- Transcriptional analysis reveals that the intracellular lipid accumulation impairs gene expression profiles involved in insulin response-associated cardiac functionality.Research square · 2023Article
- Endothelium-Dependent Hyperpolarization (EDH) in Diabetes: Mechanistic Insights and Therapeutic Implications.International journal of molecular sciences · 2019Review
- Decreased EDHF-mediated relaxation is a major mechanism in endothelial dysfunction in resistance arteries in aged mice on prolonged high-fat sucrose diet.Physiological reports · 2017Article
- Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications.Pharmacological reviews · 2017Review
- Pannexin channel and connexin hemichannel expression in vascular function and inflammation.BMC cell biology · 2017Review
- Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute to endothelial dysfunction related to insulin resistance in rats and morbidly obese humans.The Journal of physiology · 2016Article
- Vascular endothelial dysfunction and pharmacological treatment.World journal of cardiology · 2015Review
- Impact of obesity on 7,12-dimethylbenz[a]anthracene-induced altered ovarian connexin gap junction proteins in female mice.Toxicology and applied pharmacology · 2015Article
- Hepatic oxidative stress, genotoxicity and vascular dysfunction in lean or obese Zucker rats.PloS one · 2015Article
- Differential effect of amylin on endothelial-dependent vasodilation in mesenteric arteries from control and insulin resistant rats.PloS one · 2015Article
- AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is disrupted in hypertension.Science signaling · 2014Article
- Article
- Low intrinsic exercise capacity in rats predisposes to age-dependent cardiac remodeling independent of macrovascular function.American journal of physiology. Heart and circulatory physiology · 2013Article
- Advanced glycation end products acutely impair ca(2+) signaling in bovine aortic endothelial cells.Frontiers in physiology · 2013Article
- Microvascular responsiveness in obesity: implications for therapeutic intervention.British journal of pharmacology · 2012Review
- Altered gap junctional communication and renal haemodynamics in Zucker fatty rat model of type 2 diabetes.Diabetologia · 2011Article
- Diet-induced obesity impairs endothelium-derived hyperpolarization via altered potassium channel signaling mechanisms.PloS one · 2011Article
- Impairment of both nitric oxide-mediated and EDHF-type relaxation in small mesenteric arteries from rats with streptozotocin-induced diabetes.British journal of pharmacology · 2011Article
- Endothelial dysfunction in diabetes: multiple targets for treatment.Pflugers Archiv : European journal of physiology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisThe objective of this study was to examine the effect of insulin resistance on endothelium-derived hyperpolarising factor (EDHF) and small mesenteric artery endothelial function using 25-week-old insulin-resistant obese Zucker rats (OZRs) and lean littermate control rats (LZRs). The involvement of gap junctions and their connexin subunits in the EDHF relaxation response was also assessed.
methodsMesenteric arteries were evaluated using the following assays: (1) endothelial function by pressure myography, with internal diameter recorded using video microscopy; (2) connexin protein levels by western blotting; and (3) Cx mRNA expression by real-time PCR.
resultsRelaxations in response to acetylcholine were significantly smaller in mesenteric arteries from the OZRs than the LZRs, whereas there was no difference in relaxations in response to levcromakalim. Responses to acetylcholine were not altered by nitric oxide inhibitors, but were abolished by charybdotoxin in combination with apamin, which blocked the EDHF component of the response. 40Gap27 significantly attenuated the response to acetylcholine in the LZRs, but had no effect in the OZRs. Connexin 40 protein and Cx40 mRNA levels in mesenteric vascular homogenates were significantly smaller in the OZRs than in the LZRs, with no difference in connexin 43 or Cx43 mRNA levels. CONCLUSIONS/
interpretationThese findings demonstrate that endothelial dysfunction in mesenteric arteries from the insulin-resistant OZRs can be attributed to a defect in EDHF. The results also suggest that the defective EDHF is at least partly related to an impairment of connexin 40-associated gap junctions, through a decrease in connexin 40 protein and Cx40 mRNA expression in the OZRs.
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