ArticleStem cells (Dayton, Ohio)2008
Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
Article in Stem cells (Dayton, Ohio), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Controlling CRISPR-Cas9 genome editing in human cells using a molecular glue degrader.Molecular therapy. Nucleic acids · 2025Article
- Achieving Optimal Transfection Conditions in Chicken Primordial Germ Cells Under Feeder- and Serum-Free Medium.Animals : an open access journal from MDPI · 2025Article
- A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells.International journal of molecular sciences · 2023Article
- Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14.Stem cell research & therapy · 2019Article
- Bone marrow stem cells to destroy circulating HIV: a hypothetical therapeutic strategy.Journal of biological research (Thessalonike, Greece) · 2018Article
- Improved Transfection Efficiency and Metabolic Activity in Human Embryonic Stem Cell Using Non-Enzymatic Method.International journal of stem cells · 2018Article
- Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Adeno-Associated Virus Vectors and Stem Cells: Friends or Foes?Human gene therapy · 2017Review
- Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation.Journal of visualized experiments : JoVE · 2016Article
- Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency.BioResearch open access · 2016Article
- Efficient, Low-Cost Nucleofection of Passaged Chondrocytes.Cartilage · 2016Article
- Epigenetic changes of lentiviral transgenes in porcine stem cells derived from embryonic origin.PloS one · 2013Article
- Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential.Stem cells international · 2012Article
- Directing human embryonic stem cell differentiation by non-viral delivery of siRNA in 3D culture.Biomaterials · 2011Article
- Transfecting and nucleofecting human induced pluripotent stem cells.Journal of visualized experiments : JoVE · 2011Article
- Article
- Enhanced transfection efficiency of human embryonic stem cells by the incorporation of DNA liposomes in extracellular matrix.Stem cells and development · 2010Article
- High-efficiency siRNA-based gene knockdown in human embryonic stem cells.RNA (New York, N.Y.) · 2010Article
- Efficient, high-throughput transfection of human embryonic stem cells.Stem cell research & therapy · 2010Article
- mTOR-mediated activation of p70 S6K induces differentiation of pluripotent human embryonic stem cells.Cellular reprogramming · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
High-efficiency genetic modification of human embryonic stem (hES) cells would enable manipulation of gene activity, routine gene targeting, and development of new human disease models and treatments. Chemical transfection, nucleofection, and electroporation of hES cells result in low transfection efficiencies. Viral transduction is efficient but has significant drawbacks. Here we describe techniques to transiently and stably express transgenes in hES cells with high efficiency using a widely available vector system. The technique combines nucleofection of single hES cells with improved methods to select hES cells at clonal density. As validation, we reduced Oct4 and Nanog expression using siRNAs and shRNA vectors in hES cells. Furthermore, we derived many hES cell clones with either stably reduced alkaline phosphatase activity or stably overexpressed green fluorescent protein. These clones retained stem cell characteristics (normal karyotype, stem cell marker expression, self-renewal, and pluripotency). These studies will accelerate efforts to interrogate gene function and define the parameters that control growth and differentiation of hES cells. Disclosure of potential conflicts of interest is found at the end of this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.