ArticlePlant molecular biology2008
Plant Sar1 isoforms with near-identical protein sequences exhibit different localisations and effects on secretion.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 70 citations in OpenAlex.
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- Chloroplast Stress Signals Orchestrate Epidermis-Specific Remodeling of Mitochondria and ER Under High Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection.The New phytologist · 2026Article
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- Arabidopsis Sar1 isoforms play redundant roles in female gametophytic development.Plant reproduction · 2023Article
- A CYP78As-small grain4-coat protein complex Ⅱ pathway promotes grain size in rice.The Plant cell · 2023Article
- Down-Regulation of Rice Glutelin byInternational journal of molecular sciences · 2023Article
- Interactome ofInternational journal of molecular sciences · 2023Article
- A deleterious Sar1c variant in rice inhibits export of seed storage proteins from the endoplasmic reticulum.Plant molecular biology · 2023Article
- FYVE2, a phosphatidylinositol 3-phosphate effector, interacts with the COPII machinery to control autophagosome formation in Arabidopsis.The Plant cell · 2022Article
- On the nature of the plant ER exit sites.Frontiers in plant science · 2022Article
- Development of a Viral RdRp-Assisted Gene Silencing System and Its Application in the Identification of Host Factors of Plant (+)RNA Virus.Frontiers in microbiology · 2021Article
- COPII Components Sar1b and Sar1c Play Distinct Yet Interchangeable Roles in Pollen Development.Plant physiology · 2020Article
- Transport from the endoplasmic reticulum to the Golgi in plants: Where are we now?Seminars in cell & developmental biology · 2018Review
- The plant membrane surrounding powdery mildew haustoria shares properties with the endoplasmic reticulum membrane.Journal of experimental botany · 2017Article
- Maintaining the factory: the roles of the unfolded protein response in cellular homeostasis in plants.The Plant journal : for cell and molecular biology · 2017Article
- GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells.The Plant cell · 2016Article
- Subcellular localization and trafficking of phytolongins (non-SNARE longins) in the plant secretory pathway.Journal of experimental botany · 2016Article
- Unique COPII component AtSar1a/AtSec23a pair is required for the distinct function of protein ER export in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, differentiation of subdomains of the endoplasmic reticulum (ER) dedicated to protein export, the ER export sites (ERES), is influenced by the type of export-competent membrane cargo to be delivered to the Golgi. This raises a fundamental biological question: is the formation of transport intermediates at the ER for trafficking to the Golgi always regulated in the same manner? To test this, we followed the distribution and activity of two plant Sar1 isoforms. Sar1 is the small GTPase that regulates assembly of COPII (coat protein complex II) on carriers that transport secretory cargo from ER to Golgi. We show that, in contrast to a tobacco Sar1 isoform, the two Arabidopsis Sar1 GTPases were localised at ERES, independently of co-expression of Golgi-destined membrane cargo in tobacco cells. Although both isoforms labelled ERES, one was found to partition with the membrane fraction to a greater extent. The different distribution of fluorescent fusions of the two isoforms was influenced by the nature of an amino acid residue at the C-terminus of the protein, suggesting that the requirements for membrane association of the two GTPases are not equal. Furthermore, functional analyses based on the secretion of the bulk flow marker alpha-amylase indicated that over-expression of GTP-restricted mutants of the two isoforms caused different levels of ER export inhibition. These novel results indicate a functional heterogeneity among plant Sar1 isoforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.