ArticleHelicobacter2008
Helicobacter pylori genotyping and sequencing using paraffin-embedded biopsies from residents of colombian areas with contrasting gastric cancer risks.
Article in Helicobacter, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Effect of treatment failure on the CagA EPIYA motif inWorld journal of gastroenterology · 2017Trial
- DNA Methylation Predicts Progression of Human Gastric Lesions.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2015Trial
- Prevalence and correlation with clinical diseases of Helicobacter pylori cagA and vacA genotype among gastric patients from Northeast China.BioMed research international · 2014Trial
- Virulence Genes ofCanadian journal of gastroenterology & hepatology · 2022Article
- Helicobacter pylori Infection Elicits Type I Interferon Response in Human Monocytes via Toll-Like Receptor 8 Signaling.Journal of immunology research · 2022Article
- Circulating methylated THBS1 DNAs as a novel marker for predicting peritoneal dissemination in gastric cancer.Journal of clinical laboratory analysis · 2021Article
- Article
- The Prevalence of Helicobacter pylori in Estonian Bariatric Surgery Patients.International journal of molecular sciences · 2018Article
- Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran.Advanced biomedical research · 2014Article
- In vitro effect of amoxicillin and clarithromycin on the 3' region of cagA gene in Helicobacter pylori isolates.World journal of gastroenterology · 2013Article
- Use of the noninvasive entero-test in the detection of Helicobacter pylori in children in an endemic area in Colombia.Journal of pediatric gastroenterology and nutrition · 2013Article
- Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children.Helicobacter · 2012Article
- Disruption of nitric oxide signaling by Helicobacter pylori results in enhanced inflammation by inhibition of heme oxygenase-1.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Promoter DNA hypermethylation in gastric biopsies from subjects at high and low risk for gastric cancer.International journal of cancer · 2010Article
- Gastric cancer: an infectious disease.Infectious disease clinics of North America · 2010Review
- CagA C-terminal variations in Helicobacter pylori strains from Colombian patients with gastric precancerous lesions.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2010Article
- Helicobacter pylori vacA arrangement and related diseases: a retrospective study over a period of 15 years.Digestive diseases and sciences · 2009Article
- Eosinophils and mast cells in chronic gastritis: possible implications in carcinogenesis.Human pathology · 2008Article
- Natural history of Helicobacter pylori infection.Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver · 2008Article
- Detection and Genotyping ofPakistan journal of medical sciencesArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundcagA-positive and vacA s1 and m1 genotypes of Helicobacter pylori are associated with an elevated risk of gastric cancer (GC). We determined these genotypes using paraffin-embedded gastric biopsy specimens harvested from infected individuals and compared genotype distributions in two Colombian populations residing in geographic regions with a high and low incidence of GC.
methodsDNA from paraffin-embedded gastric biopsies from 107 adults was amplified using primers specific for cagA, for the cag'empty site', for the s and m alleles of vacA, and for H. pylori 16S rRNA.
resultsH. pylori infection was detected by molecular assays in 97 (90.7%) biopsies. Complete genotyping of cagA and vacA was achieved in 94 (96.9%) cases. The presence of cagA was detected in 78 of 97 cases (80.4%); when considered separately, cagA and vacA s regions were not significantly associated with a particular geographic area. The vacA m1 allele and s1m1 genotypes were more common in the area of high risk for GC (p = .037 and p = .044, respectively), while the vacA m2 allele and s2m2 genotypes were more prevalent in the low-risk area. The prevalence of the combination of cagA-positive, vacA s1m1 genotypes was 84.3% and 60.5% for high and low risk areas, respectively (p = .011).
conclusionsH. pylori cagA and vacA genotyping from paraffin-embedded gastric biopsies permitted reliable typability and discrimination. The more virulent cagA-positive s1m1 strains, as well as vacA m1 genotype, were more prevalent in high risk than in low risk areas, which may contribute to the difference in GC risk between those two regions.
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