ArticleThe Journal of biological chemistry2008
A biotin interference assay highlights two different asymmetric interaction profiles for lambda integrase arm-type binding sites in integrative versus excisive recombination.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- The Integration and Excision of CTnDOT.Microbiology spectrum · 2015Review
- The λ Integrase Site-specific Recombination Pathway.Microbiology spectrum · 2015Review
- Mapping the λ Integrase bridges in the nucleoprotein Holliday junction intermediates of viral integrative and excisive recombination.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Nucleoprotein architectures regulating the directionality of viral integration and excision.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Bacteriophage mv4 site-specific recombination: the central role of the P2 mv4Int-binding site.Journal of virology · 2014Article
- Interactions of NBU1 IntN1 and Orf2x proteins with attachment site DNA.Journal of bacteriology · 2013Article
- A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability.Molecular and cellular biology · 2011Article
- Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates.Nucleic acids research · 2010Article
- CTnDOT integrase interactions with attachment site DNA and control of directionality of the recombination reaction.Journal of bacteriology · 2010Article
- Selection of bacteriophage lambda integrases with altered recombination specificity by in vitro compartmentalization.Nucleic acids research · 2010Article
- Teaching Cre to follow directions.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A chimeric Cre recombinase with regulated directionality.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The site-specific recombinase integrase encoded by bacteriophage lambda promotes integration and excision of the viral chromosome into and out of its Escherichia coli host chromosome through a Holliday junction recombination intermediate. This intermediate contains an integrase tetramer bound via its catalytic carboxyl-terminal domains to the four "core-type" sites of the Holliday junction DNA and via its amino-terminal domains to distal "arm-type" sites. The two classes of integrase binding sites are brought into close proximity by an ensemble of accessory proteins that bind and bend the intervening DNA. We have used a biotin interference assay that probes the requirement for major groove protein binding at specified DNA loci in conjunction with DNA protection, gel mobility shift, and genetic experiments to test several predictions of the models derived from the x-ray crystal structures of minimized and symmetrized surrogates of recombination intermediates lacking the accessory proteins and their cognate DNA targets. Our data do not support the predictions of "non-canonical" DNA targets for the N-domain of integrase, and they indicate that the complexes used for x-ray crystallography are more appropriate for modeling excisive rather than integrative recombination intermediates. We suggest that the difference in the asymmetric interaction profiles of the N-domains and arm-type sites in integrative versus excisive recombinogenic complexes reflects the regulation of recombination, whereas the asymmetry of these patterns within each reaction contributes to directionality.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.