ArticlePharmaceutical research2008
Plasmid CpG depletion improves degree and duration of tumor gene expression after intravenous administration of polyplexes.
Article in Pharmaceutical research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Electroporation- and Liposome-Mediated Co-Transfection of Single and Multiple Plasmids.Pharmaceutics · 2025Article
- Polymers as Efficient Non-Viral Gene Delivery Vectors: The Role of the Chemical and Physical Architecture of Macromolecules.Polymers · 2024Review
- Construction of pVAX-1-based linear covalently closed vector with improved transgene expression.Molecular biology reports · 2024Article
- Adeno-associated virus-vectored delivery of HIV biologics: the promise of a "single-shot" functional cure for HIV infection.Journal of virus eradication · 2023Article
- Gene Therapy in the Anterior Eye Segment.Current gene therapy · 2022Review
- Review
- Limited expression of non-integrating CpG-free plasmid is associated with increased nucleosome enrichment.PloS one · 2020Article
- Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells.International journal of molecular sciences · 2017Article
- A novel micro-linear vector for in vitro and in vivo gene delivery and its application for EBV positive tumors.PloS one · 2012Article
- Effect of GPE-AGT nanoparticle shRNA transfection system mediated RNAi on early atherosclerotic lesion.International journal of clinical and experimental pathology · 2012Article
- Personalized cancer approach: using RNA interference technology.World journal of surgery · 2011Review
- Epidermal growth factor receptor-targeted (131)I-therapy of liver cancer following systemic delivery of the sodium iodide symporter gene.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.Gene therapy · 2011Article
- Plasmid injection and application of electric pulses alter endogenous mRNA and protein expression in B16.F10 mouse melanomas.Cancer gene therapy · 2010Article
- pEPito: a significantly improved non-viral episomal expression vector for mammalian cells.BMC biotechnology · 2010Article
- RNA interference for improving the outcome of islet transplantation.Advanced drug delivery reviewsReview
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
purposeTumor gene expression after the intravenous (i.v.) administration of current polymer-based gene delivery systems is generally low and short-lived. Immune stimulatory CpG dinucleotides, present within the plasmid DNA of the polyplexes are likely to contribute to this. The effect of CpG replacement on the levels of transgene expression was studied, after the i.v. administration of polyethylenimine (PEI) polyplexes.
methodsTumor transfection and immune stimulation of PEI polyplexes containing plasmid DNA encoding for luciferase and rich in CpG motifs was monitored and compared to polyplexes containing the same gene but devoid of CpG motifs. Lipoplexes based on 1,2-dioleyl-3-trimethylammonium-propane/dioleoylphosphatidylethanolamine liposomes were included as a control.
resultsThe replacement of CpGrich DNA by CpGfree DNA did neither affect the physical properties of the DNA complexes nor did it affect their in vitro transfection activity or cytotoxicity. The immune stimulation (interleukin-12) after i.v. administration of the PEI DNA complexes was low and unaffected by the presence of CpG motifs. The absence of CpG motifs within the different DNA complexes improved the degree and the duration of organ and tumor gene expression.
conclusionThe depletion of CpG dinucleotides within the plasmid DNA of polyplexes enhances the degree and duration of in vivo transgene expression.
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