ArticlePlant cell reports2008
Evaluation of selection strategies alternative to nptII in genetic transformation of citrus.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 81 citations in OpenAlex.
- Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.International journal of molecular sciences · 2024Review
- Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene.Transgenic research · 2024Article
- Optimization ofFrontiers in plant science · 2024Article
- An Improved Procedure forPlants (Basel, Switzerland) · 2022Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood' (3 Biotech · 2020Article
- New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review.Frontiers in plant science · 2020Review
- Efficient Biolistic Transformation of Immature Citrus Rootstocks Using Phosphomannose-isomerase Selection.Plants (Basel, Switzerland) · 2019Article
- Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Plants (Basel, Switzerland) · 2019Article
- Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis.Scientific reports · 2019Article
- The relationship between PMI (manA) gene expression and optimal selection pressure in Indica rice transformation.Plant cell reports · 2014Article
- Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection.Plant cell reports · 2013Article
- Genetic transformation in citrus.TheScientificWorldJournal · 2013Review
- Genetic transformation of fruit trees: current status and remaining challenges.Transgenic research · 2012Review
- Successful recovery of transgenic cowpea (Vigna unguiculata) using the 6-phosphomannose isomerase gene as the selectable marker.Plant cell reports · 2012Article
- Genetic engineering of radish: current achievements and future goals.Plant cell reports · 2011Review
- Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.Plant cell reports · 2010Review
- Combining a regeneration-promoting ipt gene and site-specific recombination allows a more efficient apricot transformation and the elimination of marker genes.Plant cell reports · 2009Article
- Evaluation of a morphological marker selection and excision system to generate marker-free transgenic cassava plants.Plant cell reports · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. The present work investigates three different selection alternatives, comparing them to nptII selection in two citrus genotypes, Carrizo citrange and Pineapple sweet orange. The first method used the beta-glucuronidase (uidA) reporter marker gene for selection; the second attempted to generate marker-free plants by transforming explants with a multi-auto-transformation (MAT) vector, combining an inducible R/RS-specific recombination system with transgenic-shoot selection through expression of isopentenyl transferase (ipt) and indoleacetamide hydrolase/tryptophan monooxygenase (iaaM/H) marker genes; while the third exploited the phosphomannose isomerase (PMI)/mannose conditional positive selection system. Firstly, GUS screening of all regenerated shoots in kanamycin-free medium gave 4.3% transformation efficiency for both genotypes. Secondly, workable transformation efficiencies were also achieved with the MAT system, 7.2% for citrange and 6.7% for sweet orange. This system affords an additional advantage as it enables selectable marker genes to be used during the in vitro culture phase and later removed from the transgenic plants by inducible recombination and site-specific excision. Thirdly, the highest transformation rates were obtained with the PMI/mannose system, 30% for citrange and 13% for sweet orange, which indicates that this marker is also an excellent candidate for citrus transformation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.