ArticlePlant cell reports2008
Development of a transient expression system in grapevine via agro-infiltration.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 128 citations in OpenAlex.
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- Dynamic temporal transcriptome analysis reveals grape VlMYB59-Horticulture research · 2024Article
- A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries.Protoplasma · 2024Article
- QTL detection and candidate gene analysis of grape white rot resistance by interspecific grape (Horticulture research · 2023Article
- An Erwinia amylovora inducible promoter for improvement of apple fire blight resistance.Plant cell reports · 2022Article
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- The PyPIF5-PymiR156a-PySPL9-PyMYB114/MYB10 module regulates light-induced anthocyanin biosynthesis in red pear.Molecular horticulture · 2021Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Transient expression of human serum albumin (HSA) in tobacco leaves.Molecular biology reports · 2020Article
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- Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method.Virusdisease · 2019Article
- Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.Frontiers in bioengineering and biotechnology · 2019Article
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- Review
- Rapid enhancement of α-tocopherol content in3 Biotech · 2018Article
- Molecular cloning of a CC-NBS-LRR gene from Vitis quinquangularis and its expression pattern in response to downy mildew pathogen infection.Molecular genetics and genomics : MGG · 2018Article
- A new image-based tool for the high throughput phenotyping of pollen viability: evaluation of inter- and intra-cultivar diversity in grapevine.Plant methods · 2018Article
- Identification of a Vitis vinifera endo-β-1,3-glucanase with antimicrobial activity against Plasmopara viticola.Molecular plant pathology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient expression of genes using Agrobacterium is a powerful tool for the analysis of gene function in plants. We have developed this method for the analysis of genes involved in disease resistance in grapevine leaves. Our research showed that the quality of the plant material, the plant genotype used for agro-infiltration and the presence of additional virulence factors (carried on plasmid pCH32) in the Agrobacterium strain are all important factors for success of the procedure. After optimising these factors, we consistently achieve sufficient acceptable levels of expression of the markers beta-glucuronidase (GUS) and green fluorescent protein (GFP) using vacuum infiltration of grapevine leaves from plants grown in vitro. We used this procedure to investigate the proposed role of stilbenes in defense against grapevine downy mildew (Plasmopara viticola) by transiently overexpressing stilbene synthase in grapevine leaves, before infection with P. viticola. We found that agro-infiltration itself induces the synthesis of stilbenes in grapevine leaves, thus preventing us to test the effect of the overexpression of stilbene synthase in defense. However, our results revealed that agro-infiltration before P. viticola inoculation had an effect on the development of the infection. Further research is required to show whether stilbenes or some other factor are the causal agent restricting pathogen development. The method described here provides and excellent tool to exploit at the many grapevine genomic resources now available, and will contribute to a better understanding of many areas of grapevine biology.
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