ArticleEuropean biophysics journal : EBJ2008
Monitoring the assembly of antibody-binding membrane protein arrays using polarised neutron reflection.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Fully Aqueous Self-Assembly of a Gold-Nanoparticle-Based Pathogen Sensor.International journal of molecular sciences · 2023Article
- Modular Protein Engineering Approach to the Functionalization of Gold Nanoparticles for Use in Clinical Diagnostics.ACS applied nano materials · 2018Article
- The effects of tether placement on antibody stability on surfaces.The Journal of chemical physics · 2017Article
- Application of advanced sampling and analysis methods to predict the structure of adsorbed protein on a material surface.Biointerphases · 2017Article
- Engineered self-assembling monolayers for label free detection of influenza nucleoprotein.Biomedical microdevices · 2015Article
- Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry.Journal of the Royal Society, Interface · 2012Article
- Self-assembly of protein monolayers engineered for improved monoclonal immunoglobulin G binding.International journal of molecular sciences · 2011Article
- Neutrons for biologists: a beginner's guide, or why you should consider using neutrons.Journal of the Royal Society, Interface · 2009Review
- Interfacial assembly of proteins and peptides: recent examples studied by neutron reflection.Journal of the Royal Society, Interface · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein arrays are used in a wide range of applications. The array described here binds IgG antibodies, produced in rabbit, to gold surfaces via a scaffold protein. The scaffold protein is a fusion of the monomeric E. coli porin outer membrane protein A (OmpA) and the Z domain of Staphylococcus aureus protein A. The OmpA binds to gold surfaces via a cysteine residue in a periplasmic turn and the Z domain binds immunoglobulins via their constant region. Polarised Neutron Reflection is used to probe the structure perpendicular to the gold surface at each stage of the assembly of the arrays. Polarised neutrons are used as this provides a means of achieving extra contrast in samples having a magnetic metal layer under the gold surface. This contrast is attained without resorting to hydrogen/deuterium exchange in the biological layer. Polarised Neutron Reflection allows for the modelling of many and complex layers with good fits. The total thickness of the biological layer immobilised on the gold surface is found to be 187 A and the layer can thus far be separated into its lipid, protein and solvent parts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.