ArticleJournal of biomedicine & biotechnology2008
Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production.
Article in Journal of biomedicine & biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed, 40 citations in OpenAlex.
- Designing an irreversible metabolic switch for scalable induction of microbial chemical production.Nature communications · 2021Article
- Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3).PloS one · 2018Article
- Heterogeneity in protein expression induces metabolic variability in a modeled Escherichia coli population.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Membrane stresses induced by overproduction of free fatty acids in Escherichia coli.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli, a long-chain acyl-CoA is a regulatory signal that modulates gene expression through its binding to a transcription factor FadR. In this study, comparative proteomic analysis of E. coli in the presence of glucose and oleic acid was performed to understand cell physiology in response to oleic acid. Among total of 52 proteins showing altered expression levels with oleic acid presence, 9 proteins including AldA, Cdd, FadA, FadB, FadL, MalE, RbsB, Udp, and YccU were newly synthesized. Among the genes that were induced by oleic acid, the promoter of the aldA gene was used for the production of a green fluorescent protein (GFP). Analysis of fluorescence intensities and confocal microscopic images revealed that soluble GFP was highly expressed under the control of the aldA promoter. These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.